Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreERT2 transgenic piglet

Eunhye Kim, Seon Ung Hwang, Junchul David Yoon, Hyunggee Kim, Gabsang Lee, Sang Hwan Hyun

Research output: Contribution to journalArticle

Abstract

BACKGROUND: The porcine brain is gyrencephalic with similar gray and white matter composition and size more comparable to the human rather than the rodent brain; however, there is lack of information about neural progenitor cells derived from this model. RESULTS: Here, we isolated GFAP-positive porcine neural stem cells (NSCs) from the brain explant of a transgenic piglet, with expression of CreERT2 under the control of the GFAP promoter (pGFAP-CreERT2). The isolated pGFAP-CreERT2 NSCs showed self-renewal and expression of representative NSC markers such as Nestin and Sox2. Pharmacological inhibition studies revealed that Notch1 signaling is necessary to maintain NSC identity, whereas serum treatment induced cell differentiation into reactive astrocytes and neurons. CONCLUSIONS: Collectively, these results indicate that GFAP promoter-driven porcine CreERT2 NSCs would be a useful tool to study neurogenesis of the porcine adult central nervous system and furthers our understanding of its potential clinical application in the future. ᅟ.

Original languageEnglish
Number of pages1
JournalBMC Veterinary Research
Volume14
Issue number1
DOIs
Publication statusPublished - 2018 Nov 7

Fingerprint

Neural Stem Cells
stem cells
piglets
Swine
Stem Cells
genetically modified organisms
swine
brain
Brain
Nestin
promoter regions
Neurogenesis
neurogenesis
Astrocytes
astrocytes
Cell Differentiation
Rodentia
cell differentiation
Central Nervous System
central nervous system

Keywords

  • Neural stem cells
  • Notch signaling
  • Pig
  • Reactive astrocytes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreERT2 transgenic piglet. / Kim, Eunhye; Hwang, Seon Ung; Yoon, Junchul David; Kim, Hyunggee; Lee, Gabsang; Hyun, Sang Hwan.

In: BMC Veterinary Research, Vol. 14, No. 1, 07.11.2018.

Research output: Contribution to journalArticle

Kim, Eunhye ; Hwang, Seon Ung ; Yoon, Junchul David ; Kim, Hyunggee ; Lee, Gabsang ; Hyun, Sang Hwan. / Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreERT2 transgenic piglet. In: BMC Veterinary Research. 2018 ; Vol. 14, No. 1.
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AU - Lee, Gabsang

AU - Hyun, Sang Hwan

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