Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells

Jai Hee Moon, Sung Sik Kwak, Gyuman Park, Hye Youn Jung, Byung Sun Yoon, Jaeyeo Park, Kyung Su Ryu, Seung Cheol Choi, Isaac Maeng, Bona Kim, Eun Kyung Jun, Soonseong Kim, Aeree Kim, Sejong Oh, Hyunggee Kim, Ki Dong Kim, Seungkwon You

Research output: Contribution to journalArticle

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Abstract

In this study, we report the isolation and characterization of a population of multipotent keloid-derived mesenchymal-like stem cells (KMLSCs) from keloid scalp tissues. These KMLSCs expressed the typical mesenchymal stem cell marker proteins CD13, CD29, CD44, CD90, fibronectin, and vimentin when they were cultured in serum-containing medium and when subsequent exposure to various differentiation media resulted in their differentiation into adipocytes, osteoblasts, chondrocytes, smooth muscle cells, and angiogenic endothelial cells. When KMLSCs were cultured in neural stem culture conditions (i.e., in the presence of epidermal growth factor and fibroblast growth factor 2 in substrate-free conditions), they produced large numbers of neurospheres containing nestin-, CD133-, and SOX2-positive cells that expressed neural-crest stem cell markers. Subsequent exposure of these cells to different differentiation conditions resulted in cells that expressed neuronal cell-, astrocyte-, oligodendrocyte-, or Schwann cell-specific markers. Our study suggests that KMLSCs may be an alternative adult stem cell resource for regenerative tissue repair and auto-transplantation.

Original languageEnglish
Pages (from-to)713-724
Number of pages12
JournalStem Cells and Development
Volume17
Issue number4
DOIs
Publication statusPublished - 2008 Aug 1

Fingerprint

Keloid
Stem cells
Mesenchymal Stromal Cells
Cells
Nestin
Adult Stem Cells
Neural Stem Cells
Neural Crest
Schwann Cells
Oligodendroglia
Vimentin
Fibroblast Growth Factor 2
Chondrocytes
Scalp
Osteoblasts
Tissue
Fibronectins
Epidermal Growth Factor
Adipocytes
Astrocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells. / Moon, Jai Hee; Kwak, Sung Sik; Park, Gyuman; Jung, Hye Youn; Yoon, Byung Sun; Park, Jaeyeo; Ryu, Kyung Su; Choi, Seung Cheol; Maeng, Isaac; Kim, Bona; Jun, Eun Kyung; Kim, Soonseong; Kim, Aeree; Oh, Sejong; Kim, Hyunggee; Kim, Ki Dong; You, Seungkwon.

In: Stem Cells and Development, Vol. 17, No. 4, 01.08.2008, p. 713-724.

Research output: Contribution to journalArticle

Moon, JH, Kwak, SS, Park, G, Jung, HY, Yoon, BS, Park, J, Ryu, KS, Choi, SC, Maeng, I, Kim, B, Jun, EK, Kim, S, Kim, A, Oh, S, Kim, H, Kim, KD & You, S 2008, 'Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells', Stem Cells and Development, vol. 17, no. 4, pp. 713-724. https://doi.org/10.1089/scd.2007.0210
Moon, Jai Hee ; Kwak, Sung Sik ; Park, Gyuman ; Jung, Hye Youn ; Yoon, Byung Sun ; Park, Jaeyeo ; Ryu, Kyung Su ; Choi, Seung Cheol ; Maeng, Isaac ; Kim, Bona ; Jun, Eun Kyung ; Kim, Soonseong ; Kim, Aeree ; Oh, Sejong ; Kim, Hyunggee ; Kim, Ki Dong ; You, Seungkwon. / Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells. In: Stem Cells and Development. 2008 ; Vol. 17, No. 4. pp. 713-724.
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