Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene

Se-Ho Park, J. H. Yoon, H. A. Cho, Y. D. Kwon, R. H. Seong, S. H. Hong, S. D. Park

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11 Citations (Scopus)

Abstract

A genomic DNA clone containing the 5'-end of the rat topo IIα gene was isolated and the intron/exon structure of a 4.0 kb region encompassing the translation initiation site was determined. Multiple transcription initiation sites were found at positions -128, -110, and -100 bp upstream of the ATG codon. A minimal promoter region extending from -192 to the translation initiation codon was identified on transient expression analysis. This region lacks a TATA motif, is moderately GC-rich and contains a high number of CpG dinucleotides, which is characteristic of a housekeeping gene promoter. The fragment extending to position -242 exhibited maximal promoter activity. Putative regulatory elements were delineated within and immediately upstream of the minimal promoter region. On gel retardation and DNase I footprint analyses, two regions, between positions -195 to -159 which interact with protein factor(s) were identified. The minimal promoter region of the rat topo IIα gene showed high sequence homology with that of human topo IIα. In a 250 bp region upstream of the translation initiation site, the sequence identity was about 70%. The basic structure of the regulatory region of the rat topo IIα gene was found to be similar to that of the human counterpart.

Original languageEnglish
Pages (from-to)725-733
Number of pages9
JournalJournal of Biochemistry
Volume118
Issue number4
Publication statusPublished - 1995 Jan 1
Externally publishedYes

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Keywords

  • DNA topoisomerase IIα
  • Promoter
  • Rat
  • Transcription start site
  • Transient expression

ASJC Scopus subject areas

  • Biochemistry
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology

Cite this

Park, S-H., Yoon, J. H., Cho, H. A., Kwon, Y. D., Seong, R. H., Hong, S. H., & Park, S. D. (1995). Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene. Journal of Biochemistry, 118(4), 725-733.