Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene

Se-Ho Park, J. H. Yoon, H. A. Cho, Y. D. Kwon, R. H. Seong, S. H. Hong, S. D. Park

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A genomic DNA clone containing the 5'-end of the rat topo IIα gene was isolated and the intron/exon structure of a 4.0 kb region encompassing the translation initiation site was determined. Multiple transcription initiation sites were found at positions -128, -110, and -100 bp upstream of the ATG codon. A minimal promoter region extending from -192 to the translation initiation codon was identified on transient expression analysis. This region lacks a TATA motif, is moderately GC-rich and contains a high number of CpG dinucleotides, which is characteristic of a housekeeping gene promoter. The fragment extending to position -242 exhibited maximal promoter activity. Putative regulatory elements were delineated within and immediately upstream of the minimal promoter region. On gel retardation and DNase I footprint analyses, two regions, between positions -195 to -159 which interact with protein factor(s) were identified. The minimal promoter region of the rat topo IIα gene showed high sequence homology with that of human topo IIα. In a 250 bp region upstream of the translation initiation site, the sequence identity was about 70%. The basic structure of the regulatory region of the rat topo IIα gene was found to be similar to that of the human counterpart.

Original languageEnglish
Pages (from-to)725-733
Number of pages9
JournalJournal of Biochemistry
Volume118
Issue number4
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Fingerprint

Type II DNA Topoisomerase
Genetic Promoter Regions
Promoter
Isolation
Rats
DNA
Genes
Gene
Initiator Codon
Nucleic Acid Regulatory Sequences
Transcription Initiation Site
Deoxyribonuclease I
Essential Genes
Sequence Homology
Codon
Introns
Exons
Transcription
Clone Cells
Gels

Keywords

  • DNA topoisomerase IIα
  • Promoter
  • Rat
  • Transcription start site
  • Transient expression

ASJC Scopus subject areas

  • Biochemistry
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology

Cite this

Park, S-H., Yoon, J. H., Cho, H. A., Kwon, Y. D., Seong, R. H., Hong, S. H., & Park, S. D. (1995). Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene. Journal of Biochemistry, 118(4), 725-733.

Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene. / Park, Se-Ho; Yoon, J. H.; Cho, H. A.; Kwon, Y. D.; Seong, R. H.; Hong, S. H.; Park, S. D.

In: Journal of Biochemistry, Vol. 118, No. 4, 01.01.1995, p. 725-733.

Research output: Contribution to journalArticle

Park, S-H, Yoon, JH, Cho, HA, Kwon, YD, Seong, RH, Hong, SH & Park, SD 1995, 'Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene', Journal of Biochemistry, vol. 118, no. 4, pp. 725-733.
Park S-H, Yoon JH, Cho HA, Kwon YD, Seong RH, Hong SH et al. Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene. Journal of Biochemistry. 1995 Jan 1;118(4):725-733.
Park, Se-Ho ; Yoon, J. H. ; Cho, H. A. ; Kwon, Y. D. ; Seong, R. H. ; Hong, S. H. ; Park, S. D. / Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene. In: Journal of Biochemistry. 1995 ; Vol. 118, No. 4. pp. 725-733.
@article{963532fa97de4c92bbd97ed8f73098e2,
title = "Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene",
abstract = "A genomic DNA clone containing the 5'-end of the rat topo IIα gene was isolated and the intron/exon structure of a 4.0 kb region encompassing the translation initiation site was determined. Multiple transcription initiation sites were found at positions -128, -110, and -100 bp upstream of the ATG codon. A minimal promoter region extending from -192 to the translation initiation codon was identified on transient expression analysis. This region lacks a TATA motif, is moderately GC-rich and contains a high number of CpG dinucleotides, which is characteristic of a housekeeping gene promoter. The fragment extending to position -242 exhibited maximal promoter activity. Putative regulatory elements were delineated within and immediately upstream of the minimal promoter region. On gel retardation and DNase I footprint analyses, two regions, between positions -195 to -159 which interact with protein factor(s) were identified. The minimal promoter region of the rat topo IIα gene showed high sequence homology with that of human topo IIα. In a 250 bp region upstream of the translation initiation site, the sequence identity was about 70{\%}. The basic structure of the regulatory region of the rat topo IIα gene was found to be similar to that of the human counterpart.",
keywords = "DNA topoisomerase IIα, Promoter, Rat, Transcription start site, Transient expression",
author = "Se-Ho Park and Yoon, {J. H.} and Cho, {H. A.} and Kwon, {Y. D.} and Seong, {R. H.} and Hong, {S. H.} and Park, {S. D.}",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "118",
pages = "725--733",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Isolation and characterization of the promoter region of the rat DNA topoisomerase IIα gene

AU - Park, Se-Ho

AU - Yoon, J. H.

AU - Cho, H. A.

AU - Kwon, Y. D.

AU - Seong, R. H.

AU - Hong, S. H.

AU - Park, S. D.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - A genomic DNA clone containing the 5'-end of the rat topo IIα gene was isolated and the intron/exon structure of a 4.0 kb region encompassing the translation initiation site was determined. Multiple transcription initiation sites were found at positions -128, -110, and -100 bp upstream of the ATG codon. A minimal promoter region extending from -192 to the translation initiation codon was identified on transient expression analysis. This region lacks a TATA motif, is moderately GC-rich and contains a high number of CpG dinucleotides, which is characteristic of a housekeeping gene promoter. The fragment extending to position -242 exhibited maximal promoter activity. Putative regulatory elements were delineated within and immediately upstream of the minimal promoter region. On gel retardation and DNase I footprint analyses, two regions, between positions -195 to -159 which interact with protein factor(s) were identified. The minimal promoter region of the rat topo IIα gene showed high sequence homology with that of human topo IIα. In a 250 bp region upstream of the translation initiation site, the sequence identity was about 70%. The basic structure of the regulatory region of the rat topo IIα gene was found to be similar to that of the human counterpart.

AB - A genomic DNA clone containing the 5'-end of the rat topo IIα gene was isolated and the intron/exon structure of a 4.0 kb region encompassing the translation initiation site was determined. Multiple transcription initiation sites were found at positions -128, -110, and -100 bp upstream of the ATG codon. A minimal promoter region extending from -192 to the translation initiation codon was identified on transient expression analysis. This region lacks a TATA motif, is moderately GC-rich and contains a high number of CpG dinucleotides, which is characteristic of a housekeeping gene promoter. The fragment extending to position -242 exhibited maximal promoter activity. Putative regulatory elements were delineated within and immediately upstream of the minimal promoter region. On gel retardation and DNase I footprint analyses, two regions, between positions -195 to -159 which interact with protein factor(s) were identified. The minimal promoter region of the rat topo IIα gene showed high sequence homology with that of human topo IIα. In a 250 bp region upstream of the translation initiation site, the sequence identity was about 70%. The basic structure of the regulatory region of the rat topo IIα gene was found to be similar to that of the human counterpart.

KW - DNA topoisomerase IIα

KW - Promoter

KW - Rat

KW - Transcription start site

KW - Transient expression

UR - http://www.scopus.com/inward/record.url?scp=0028848218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028848218&partnerID=8YFLogxK

M3 - Article

C2 - 8576085

AN - SCOPUS:0028848218

VL - 118

SP - 725

EP - 733

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 4

ER -