Isolation and expansion of synovial CD34-CD44 +CD90+ mesenchymal stem cells: Comparison of an enzymatic method and a direct explant technique

Dae Hee Lee, Seong Dae Joo, Seung Beom Han, Jisu Im, Soon-Hyuck Lee, Chung Hee Sonn, Kyung-Mi Lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Synovium-derived mesenchymal stem cells (MSCs) offer a promising therapeutic option for cartilage regeneration. The conventional method of MSC isolation involves single-cell suspensions using collagenases. Recently, a nonenzymatic explant technique was developed to isolate MSCs. We compared these techniques in the isolation of functional MSCs. MSCs were isolated from human fibrous and adipose synovium of osteoarthritic patients using explants or enzymatic methods. Total cell number, percentage of MSCs, and surface marker expression of MSCs were measured following expansion. Multipotentiality was determined using a MSC functional identification kit. MSCs isolated from fibrous or adipose synovium using these two techniques expressed similar levels of the surface markers CD44, CD90, and CD105, and displayed similar multipotentiality in generating adipocytes, osteoblasts, and chondrocytes. Total cell number and number of CD34-CD44+CD90+ MSCs after 10-day expansion were similar in each culture, regardless of the source and method used, although the percentage of MSCs was slightly higher in explant cultures. There were no correlations between MSC yield and patient age, Hospital for Special Surgery score, and degree of deformity under all culture conditions. Both the enzymatic and explant techniques yielded similar yields of MSCs with similar characteristics. Because the explant technique is simpler and less invasive, it may be preferred over enzymatic techniques for isolating MSCs from the synovium of osteoarthritic patients for cartilage regeneration.

Original languageEnglish
Pages (from-to)226-234
Number of pages9
JournalConnective Tissue Research
Volume52
Issue number3
DOIs
Publication statusPublished - 2011 Jun 1

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Synovial Membrane
Cartilage
Regeneration
Cell Count
Special Hospital
Cell Separation
Osteoblasts
Collagenases
Chondrocytes
Cell culture
Adipocytes
Surgery
Suspensions

Keywords

  • isolation method
  • mesenchymal stem cell
  • osteoarthritis
  • synovium

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Isolation and expansion of synovial CD34-CD44 +CD90+ mesenchymal stem cells : Comparison of an enzymatic method and a direct explant technique. / Lee, Dae Hee; Joo, Seong Dae; Han, Seung Beom; Im, Jisu; Lee, Soon-Hyuck; Sonn, Chung Hee; Lee, Kyung-Mi.

In: Connective Tissue Research, Vol. 52, No. 3, 01.06.2011, p. 226-234.

Research output: Contribution to journalArticle

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