Isolation and identification of a variant strain of Pseudomonas aeruginosa PR3 with enhanced production of 7,10-dihydroxy-8(E)-octadecenoic acid

Joel B. Ellamar, In-Hwan Kim, Ching T. Hou, Hee Su Park, Hak Ryul Kim

Research output: Contribution to journal › Article

Abstract

Oxylipins are known to have several biological functions. The oxylipin 7,10-dihydroxy-8(E)-octadecenoic acid (DOD), produced from oleic acid by Pseudomonas aeruginosa, was reported to have strong antibacterial activities against broad range of food-borne and plant pathogenic bacteria. Among DOD producers, P. aeruginosa PR3 shows efficient production of DOD from oleic acid, triolein, and olive oil. In this study, we report the serendipitous isolation and characterization of a variant strain of PR3, which upon examination showed higher efficiency for DOD production from olive oil than the original PR3 strain. Compared with the PR3 strain, the variant strain displayed enhanced DOD production by 47.6% and different carbon requirements for maximum DOD production. Considering the advantageous differences seen in this study, we propose the further characterization of this new strain and optimization of culture conditions for efficient DOD production.

Original languageEnglish
Article number101068
JournalBiocatalysis and Agricultural Biotechnology
Volume18
DOIs
Publication statusPublished - 2019 Mar 1

Fingerprint

Oxylipins
Oleic Acid
Pseudomonas aeruginosa
Triolein
Edible Plants
Acids
acids
oxylipins
Olive oil
Oleic acid
Carbon
olive oil
oleic acid
Bacteria
triolein
plant pathogenic bacteria
7,10-dihydroxy-8-octadecenoic acid
Olive Oil
carbon

Keywords

  • Bioconversion
  • Dihydroxy fatty acid
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

Cite this

Isolation and identification of a variant strain of Pseudomonas aeruginosa PR3 with enhanced production of 7,10-dihydroxy-8(E)-octadecenoic acid. / Ellamar, Joel B.; Kim, In-Hwan; Hou, Ching T.; Park, Hee Su; Kim, Hak Ryul.

In: Biocatalysis and Agricultural Biotechnology, Vol. 18, 101068, 01.03.2019.

Research output: Contribution to journal › Article

Ellamar, JB, Kim, I-H, Hou, CT, Park, HS & Kim, HR 2019, 'Isolation and identification of a variant strain of Pseudomonas aeruginosa PR3 with enhanced production of 7,10-dihydroxy-8(E)-octadecenoic acid', Biocatalysis and Agricultural Biotechnology, vol. 18, 101068. https://doi.org/10.1016/j.bcab.2019.101068
Ellamar JB, Kim I-H, Hou CT, Park HS, Kim HR. Isolation and identification of a variant strain of Pseudomonas aeruginosa PR3 with enhanced production of 7,10-dihydroxy-8(E)-octadecenoic acid. Biocatalysis and Agricultural Biotechnology. 2019 Mar 1;18. 101068. https://doi.org/10.1016/j.bcab.2019.101068
Ellamar, Joel B. ; Kim, In-Hwan ; Hou, Ching T. ; Park, Hee Su ; Kim, Hak Ryul. / Isolation and identification of a variant strain of Pseudomonas aeruginosa PR3 with enhanced production of 7,10-dihydroxy-8(E)-octadecenoic acid. In: Biocatalysis and Agricultural Biotechnology. 2019 ; Vol. 18.
@article{31652a1daded45568478826d4818ca74,
title = "Isolation and identification of a variant strain of Pseudomonas aeruginosa PR3 with enhanced production of 7,10-dihydroxy-8(E)-octadecenoic acid",
abstract = "Oxylipins are known to have several biological functions. The oxylipin 7,10-dihydroxy-8(E)-octadecenoic acid (DOD), produced from oleic acid by Pseudomonas aeruginosa, was reported to have strong antibacterial activities against broad range of food-borne and plant pathogenic bacteria. Among DOD producers, P. aeruginosa PR3 shows efficient production of DOD from oleic acid, triolein, and olive oil. In this study, we report the serendipitous isolation and characterization of a variant strain of PR3, which upon examination showed higher efficiency for DOD production from olive oil than the original PR3 strain. Compared with the PR3 strain, the variant strain displayed enhanced DOD production by 47.6{\%} and different carbon requirements for maximum DOD production. Considering the advantageous differences seen in this study, we propose the further characterization of this new strain and optimization of culture conditions for efficient DOD production.",
keywords = "Bioconversion, Dihydroxy fatty acid, Pseudomonas aeruginosa",
author = "Ellamar, {Joel B.} and In-Hwan Kim and Hou, {Ching T.} and Park, {Hee Su} and Kim, {Hak Ryul}",
year = "2019",
month = "3",
day = "1",
doi = "10.1016/j.bcab.2019.101068",
language = "English",
volume = "18",
journal = "Biocatalysis and Agricultural Biotechnology",
issn = "1878-8181",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Isolation and identification of a variant strain of Pseudomonas aeruginosa PR3 with enhanced production of 7,10-dihydroxy-8(E)-octadecenoic acid

AU - Ellamar, Joel B.

AU - Kim, In-Hwan

AU - Hou, Ching T.

AU - Park, Hee Su

AU - Kim, Hak Ryul

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Oxylipins are known to have several biological functions. The oxylipin 7,10-dihydroxy-8(E)-octadecenoic acid (DOD), produced from oleic acid by Pseudomonas aeruginosa, was reported to have strong antibacterial activities against broad range of food-borne and plant pathogenic bacteria. Among DOD producers, P. aeruginosa PR3 shows efficient production of DOD from oleic acid, triolein, and olive oil. In this study, we report the serendipitous isolation and characterization of a variant strain of PR3, which upon examination showed higher efficiency for DOD production from olive oil than the original PR3 strain. Compared with the PR3 strain, the variant strain displayed enhanced DOD production by 47.6% and different carbon requirements for maximum DOD production. Considering the advantageous differences seen in this study, we propose the further characterization of this new strain and optimization of culture conditions for efficient DOD production.

AB - Oxylipins are known to have several biological functions. The oxylipin 7,10-dihydroxy-8(E)-octadecenoic acid (DOD), produced from oleic acid by Pseudomonas aeruginosa, was reported to have strong antibacterial activities against broad range of food-borne and plant pathogenic bacteria. Among DOD producers, P. aeruginosa PR3 shows efficient production of DOD from oleic acid, triolein, and olive oil. In this study, we report the serendipitous isolation and characterization of a variant strain of PR3, which upon examination showed higher efficiency for DOD production from olive oil than the original PR3 strain. Compared with the PR3 strain, the variant strain displayed enhanced DOD production by 47.6% and different carbon requirements for maximum DOD production. Considering the advantageous differences seen in this study, we propose the further characterization of this new strain and optimization of culture conditions for efficient DOD production.

KW - Bioconversion

KW - Dihydroxy fatty acid

KW - Pseudomonas aeruginosa

UR - http://www.scopus.com/inward/record.url?scp=85062369751&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062369751&partnerID=8YFLogxK

U2 - 10.1016/j.bcab.2019.101068

DO - 10.1016/j.bcab.2019.101068

M3 - Article

AN - SCOPUS:85062369751

VL - 18

JO - Biocatalysis and Agricultural Biotechnology

JF - Biocatalysis and Agricultural Biotechnology

SN - 1878-8181

M1 - 101068

ER -

Access to Document