Isolation and in Vivo and in Vitro Antifungal Activity of Phenylacetic Acid and Sodium Phenylacetate from Streptomyces humidus

Byung Kook Hwang, Song Won Lim, Beom Seok Kim, Jung Yeop Lee, Surk Sik Moon

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The antifungal substances SH-1 and SH-2 were isolated from Streptomyces humidus strain S5-55 cultures by various purification procedures and identified as phenylacetic acid and sodium phenylacetate, respectively, based on the nuclear magnetic resonance, electron ionization mass spectral, and inductively coupled plasma mass spectral data. SH-1 and SH-2 completely inhibited the growth of Pythium ultimum, Phytophthora capsici, Rhizoctonia solani, Saccharomyces cerevisiae, and Pseudomonas syringae pv. syringae at concentrations from 10 to 50 μg/ml. The two compounds were as effective as the commercial fungicide metalaxyl in inhibiting spore germination and hyphal growth of P. capsici. However, the in vivo control efficacies of the two antifungal compounds against P. capsici infection on pepper plants were similar to those of H3PO3 and fosetyl-Al but less than that of metalaxyl.

Original languageEnglish
Pages (from-to)3739-3745
Number of pages7
JournalApplied and Environmental Microbiology
Issue number8
Publication statusPublished - 2001 Aug 1


ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

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