Isolation of 151 mutants that have developmental defects from T-DNA tagging

Ji Hoon Ahn, Joonki Kim, Seong Jeon Yoo, So Yeon Yoo, Hyungmin Roh, Jun Hyuk Choi, Mi Suk Choi, Kyung Sook Chung, Eun Ju Han, Sung Myun Hong, Sung Hye Jung, Hyo Jin Kang, Bo Kyung Kim, Mi Duk Kim, Youn Kyung Kim, Yun Hee Kim, Hanna Lee, Soo Hyun Park, Jee Hoon Yang, Jung Won YangDong Hun Yoo, Seung Kwan Yoo, Jong Seob Lee

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Abstract

In order to understand the mechanisms underlying plant development, a necessary first step involves the elucidation of the functions of the genes, via the analysis of mutants that exhibit developmental defects. In this study, an activation tagging mutant library harboring 80,650 independent Arabidopsis transformants was generated in order to screen for developmental mutants. A total of 129 mutants manifesting dominant developmental abnormalities were isolated, and their T-DNA insertion loci were mapped. The activation of one or more genes adjacent to a T-DNA insertion locus was confirmed in eight dominant mutants. A gene adjacent to the right border was usually activated by the 35S enhancers. Interestingly, the transcriptional activation of multiple genes within a broad range was observed in one of the mutants, which raises the possibility that activation by the 35S enhancers was not limited strictly to a single gene. In order to gain a better understanding of sexual reproduction in higher plants, we isolated 22 mutants exhibiting defects in female gametophyte development, and determined their T-DNA insertion loci. We propose that this mutant population may prove useful in the further determination of the functions of genes that play important roles in plant development.

Original languageEnglish
Pages (from-to)169-178
Number of pages10
JournalPlant and Cell Physiology
Volume48
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

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Keywords

  • Activation tagging
  • Arabidopsis
  • Developmental mutants
  • Female gametophytic mutant
  • Plant development

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Ahn, J. H., Kim, J., Yoo, S. J., Yoo, S. Y., Roh, H., Choi, J. H., Choi, M. S., Chung, K. S., Han, E. J., Hong, S. M., Jung, S. H., Kang, H. J., Kim, B. K., Kim, M. D., Kim, Y. K., Kim, Y. H., Lee, H., Park, S. H., Yang, J. H., ... Lee, J. S. (2007). Isolation of 151 mutants that have developmental defects from T-DNA tagging. Plant and Cell Physiology, 48(1), 169-178. https://doi.org/10.1093/pcp/pcl052