Isolation of a 20-kDa cAMP-dependent phosphoprotein and its putative gene pp20 in Drosophila melanogaster

Nam Gon Lee, Ga Hee Choi, Dong Hwan Won, Dongho Geum, Joonki Kim, Kyoung Sang Cho

Research output: Contribution to journalArticle

Abstract

Although the catalytic subunit of protein kinase A (PKA) is preferentially expressed in the male reproductive organs of Drosophila, the targets of PKA in these organs are poorly studied. Previously, we found three putative PKA substrates (pp45, pp20, and pp10) in the male accessory glands of D, melanogaster. Here, we partially purified pp20 and determined the amino acid sequence of a 12-residue region. A GenBank search based on this partial sequence revealed that this is a novel protein that has not been identified or annotated. A tBLASTn search based on the 12 identified residues yielded a genomic region that putatively encoded the pp20 protein, and an expressed sequence tag (EST) search revealed an EST whose sequence corresponded to that around this genomic region. Using RACE-PCR with this EST sequence, we isolated a full-length cDNA and its putative gene, which we called pp20. The product of pp20 contained all 12 residues identified in pp20, which strongly suggests that pp20 encodes pp20. Interestingly, the expression of pp20 was detected not only in adult male accessory glands but also in all developmental stages, implying that it has biological functions during development in addition to male reproduction.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalKorean Journal of Genetics
Volume29
Issue number2
Publication statusPublished - 2007 Jun 1

Fingerprint

Phosphoproteins
Expressed Sequence Tags
Drosophila melanogaster
Genes
Accessories
Cyclic AMP-Dependent Protein Kinases
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
Drosophila Proteins
Nucleic Acid Databases
Proteins
Complementary DNA
Reproduction
Amino Acids
Amino Acid Sequence
Substrates
Polymerase Chain Reaction

Keywords

  • 20-kDa cAMP-dependant phosphoprotein
  • Drosophila
  • Male accessory glands
  • pp20
  • Protein kinase A

ASJC Scopus subject areas

  • Genetics

Cite this

Isolation of a 20-kDa cAMP-dependent phosphoprotein and its putative gene pp20 in Drosophila melanogaster. / Lee, Nam Gon; Choi, Ga Hee; Won, Dong Hwan; Geum, Dongho; Kim, Joonki; Cho, Kyoung Sang.

In: Korean Journal of Genetics, Vol. 29, No. 2, 01.06.2007, p. 161-169.

Research output: Contribution to journalArticle

Lee, Nam Gon ; Choi, Ga Hee ; Won, Dong Hwan ; Geum, Dongho ; Kim, Joonki ; Cho, Kyoung Sang. / Isolation of a 20-kDa cAMP-dependent phosphoprotein and its putative gene pp20 in Drosophila melanogaster. In: Korean Journal of Genetics. 2007 ; Vol. 29, No. 2. pp. 161-169.
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