Isolation of antioxidant with exercise enhancing potential from pseudosasa japonica leaves

Yanghee You, Kyungmi Kim, Jeongmin Lee, Kwontack Hwang, Kwang Won Lee, Jin Woong Chung, Sangin Shim, Woojin Jun

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An antioxidative compound with effective exercise-enhancing potential was isolated from the ethanolic extract of Pseudosasa japonica leaves. The ethanolic extract was partitioned in the order of hexane, chloroform, ethyl acetate, and water. Of the 4 fractions, the ethyl acetate-fraction (PJE-E) showed profound scavenging activity on superoxide anion- and ABTs cation radicals. Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) were conducted to isolate a compound with antioxidative activity on exercise endurance capacity from the PJE-E. The structure of the resulting compound was identified as ferulic acid by 1H/13C nuclear magnetic resonance (NMR). This ferulic acid was orally administered to mice for 18 days and its effect on exercise endurance capacity was investigated using an adjustablecurrent water pool. Compared to the control group, a 1.8 fold increase in swimming time was observed in the ferulic acid-administered mice. Results indicate that ferulic acid from P. japonica leaves might contribute to the potent exercise-enhancing effect.

Original languageEnglish
Pages (from-to)571-574
Number of pages4
JournalFood Science and Biotechnology
Volume19
Issue number2
DOIs
Publication statusPublished - 2010 Apr 1

Fingerprint

ferulic acid
Pseudosasa
exercise
Antioxidants
antioxidants
leaves
Water
mice
extracts
Hexanes
Thin Layer Chromatography
Chloroform
thin layer chromatography
Superoxides
chloroform
superoxide anion
hexane
Cations
nuclear magnetic resonance spectroscopy
cations

Keywords

  • Antioxidant
  • Exercise endurance capacity
  • Ferulic acid
  • Pseudosasa japonica

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Isolation of antioxidant with exercise enhancing potential from pseudosasa japonica leaves. / You, Yanghee; Kim, Kyungmi; Lee, Jeongmin; Hwang, Kwontack; Lee, Kwang Won; Chung, Jin Woong; Shim, Sangin; Jun, Woojin.

In: Food Science and Biotechnology, Vol. 19, No. 2, 01.04.2010, p. 571-574.

Research output: Contribution to journalArticle

You, Yanghee ; Kim, Kyungmi ; Lee, Jeongmin ; Hwang, Kwontack ; Lee, Kwang Won ; Chung, Jin Woong ; Shim, Sangin ; Jun, Woojin. / Isolation of antioxidant with exercise enhancing potential from pseudosasa japonica leaves. In: Food Science and Biotechnology. 2010 ; Vol. 19, No. 2. pp. 571-574.
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