Isolation of DNA using magnetic nanoparticles coated with dimercaptosuccinic acid

Ji Hyun Min, Mi Kyung Woo, Ha Young Yoon, Jin Woo Jang, Jun Hua Wu, Chae Seung Lim, Young-geun Kim

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Lately, the isolation of DNA using magnetic nanoparticles has received increased attention owing to their facile manipulation and low costs. Although methods involving their magnetic separation have been extensively studied, there is currently a need for an efficient technique to isolate DNA for highly sensitive diagnostic applications. We describe herein a method to isolate and purify DNA using biofunctionalized superparamagnetic nanoparticles synthesized by a modified polyol method to obtain the desired monodispersity, followed by surface modification with meso-2,3-dimercaptosuccinic acid (DMSA) containing carboxyl groups for DNA absorption. The DMSA-coated magnetic nanoparticles (DMSA-MNPs) were used for the isolation of DNA, with a maximum yield of 86.16%. In particular, we found that the isolation of DNA using small quantities of DMSA-MNPs was much more efficient than that using commercial microbeads (NucliSENS-easyMAG, BioMérieux). Moreover, the DMSA-MNPs were successfully employed in the isolation of genomic DNA from human blood. In addition, the resulting DNA-nanoparticle complex was directly subjected to PCR amplification without prior elution, which could eventually lead to simple, rapid, sensitive and integrated diagnostic systems.

Original languageEnglish
Pages (from-to)114-118
Number of pages5
JournalAnalytical Biochemistry
Volume447
Issue number1
DOIs
Publication statusPublished - 2014 Feb 15

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Succimer
Nanoparticles
DNA
Magnetic separation
Microspheres
Amplification
Surface treatment
Blood
Costs and Cost Analysis
Polymerase Chain Reaction

Keywords

  • Dimercaptosuccinic acid
  • DNA isolation
  • Magnetic nanoparticle
  • Superparamagnetism

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Isolation of DNA using magnetic nanoparticles coated with dimercaptosuccinic acid. / Min, Ji Hyun; Woo, Mi Kyung; Yoon, Ha Young; Jang, Jin Woo; Wu, Jun Hua; Lim, Chae Seung; Kim, Young-geun.

In: Analytical Biochemistry, Vol. 447, No. 1, 15.02.2014, p. 114-118.

Research output: Contribution to journalArticle

Min, Ji Hyun ; Woo, Mi Kyung ; Yoon, Ha Young ; Jang, Jin Woo ; Wu, Jun Hua ; Lim, Chae Seung ; Kim, Young-geun. / Isolation of DNA using magnetic nanoparticles coated with dimercaptosuccinic acid. In: Analytical Biochemistry. 2014 ; Vol. 447, No. 1. pp. 114-118.
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