Abstract
Recent epidemiological studies suggest that alcohol consumption is one of the risk factors leading to type 2 diabetes, but the direct effect of ethanol on β-cell gene expression is not known. Here, using cDNA RDA method, we isolated 43 ethanol-induced genes in pancreatic β-cells, and confirmed their differential expression by Northern blot or semi-quantitative RT-PCR. These genes were further categorized by the functional criteria based on the published data; Translation, Transcription, Metabolism, Signal transduction, Transport, Structure, Cytoskeleton, Regulation, or Putative/Unknown genes. The effects of each gene on β-cell function need to be further investigated, however, the present data strongly suggest that these genes might be related to the metabolic alterations caused by ethanol as indicated in earlier study. In particular, RPS3 gene expression was increased by ethanol, glucosamine, and cytokines, implying that ethanol might decrease the metabolic activity by oxidative stress in β-cells. Therefore, cloning of these genes in full-length and the detailed studies of each gene on β-cell functions might provide clues on the pathophysiology of type 2 diabetes caused by alcohol.
| Original language | English |
|---|---|
| Pages (from-to) | 36-42 |
| Number of pages | 7 |
| Journal | Experimental and Molecular Medicine |
| Volume | 36 |
| Issue number | 1 |
| Publication status | Published - 2004 Feb 29 |
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Keywords
- Differential expression
- Ethanol
- Pancreatic β-cell
- RDA
- Type 2 diabetes
ASJC Scopus subject areas
- Biochemistry
- Genetics
Cite this
Isolation of ethanol-induced genes in pancreatic β-cells by representational difference analysis (RDA). / Shin, Jun Seop; Kwon, Young Sam; Lee, Jae Jeong; Kim, Chan Wha.
In: Experimental and Molecular Medicine, Vol. 36, No. 1, 29.02.2004, p. 36-42.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of ethanol-induced genes in pancreatic β-cells by representational difference analysis (RDA)
AU - Shin, Jun Seop
AU - Kwon, Young Sam
AU - Lee, Jae Jeong
AU - Kim, Chan Wha
PY - 2004/2/29
Y1 - 2004/2/29
N2 - Recent epidemiological studies suggest that alcohol consumption is one of the risk factors leading to type 2 diabetes, but the direct effect of ethanol on β-cell gene expression is not known. Here, using cDNA RDA method, we isolated 43 ethanol-induced genes in pancreatic β-cells, and confirmed their differential expression by Northern blot or semi-quantitative RT-PCR. These genes were further categorized by the functional criteria based on the published data; Translation, Transcription, Metabolism, Signal transduction, Transport, Structure, Cytoskeleton, Regulation, or Putative/Unknown genes. The effects of each gene on β-cell function need to be further investigated, however, the present data strongly suggest that these genes might be related to the metabolic alterations caused by ethanol as indicated in earlier study. In particular, RPS3 gene expression was increased by ethanol, glucosamine, and cytokines, implying that ethanol might decrease the metabolic activity by oxidative stress in β-cells. Therefore, cloning of these genes in full-length and the detailed studies of each gene on β-cell functions might provide clues on the pathophysiology of type 2 diabetes caused by alcohol.
AB - Recent epidemiological studies suggest that alcohol consumption is one of the risk factors leading to type 2 diabetes, but the direct effect of ethanol on β-cell gene expression is not known. Here, using cDNA RDA method, we isolated 43 ethanol-induced genes in pancreatic β-cells, and confirmed their differential expression by Northern blot or semi-quantitative RT-PCR. These genes were further categorized by the functional criteria based on the published data; Translation, Transcription, Metabolism, Signal transduction, Transport, Structure, Cytoskeleton, Regulation, or Putative/Unknown genes. The effects of each gene on β-cell function need to be further investigated, however, the present data strongly suggest that these genes might be related to the metabolic alterations caused by ethanol as indicated in earlier study. In particular, RPS3 gene expression was increased by ethanol, glucosamine, and cytokines, implying that ethanol might decrease the metabolic activity by oxidative stress in β-cells. Therefore, cloning of these genes in full-length and the detailed studies of each gene on β-cell functions might provide clues on the pathophysiology of type 2 diabetes caused by alcohol.
KW - Differential expression
KW - Ethanol
KW - Pancreatic β-cell
KW - RDA
KW - Type 2 diabetes
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M3 - Article
VL - 36
SP - 36
EP - 42
JO - Experimental and Molecular Medicine
JF - Experimental and Molecular Medicine
SN - 1226-3613
IS - 1
ER -