Isolation of ethanol-induced genes in pancreatic β-cells by representational difference analysis (RDA)

Jun Seop Shin, Young Sam Kwon, Jae Jeong Lee, Chan Wha Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recent epidemiological studies suggest that alcohol consumption is one of the risk factors leading to type 2 diabetes, but the direct effect of ethanol on β-cell gene expression is not known. Here, using cDNA RDA method, we isolated 43 ethanol-induced genes in pancreatic β-cells, and confirmed their differential expression by Northern blot or semi-quantitative RT-PCR. These genes were further categorized by the functional criteria based on the published data; Translation, Transcription, Metabolism, Signal transduction, Transport, Structure, Cytoskeleton, Regulation, or Putative/Unknown genes. The effects of each gene on β-cell function need to be further investigated, however, the present data strongly suggest that these genes might be related to the metabolic alterations caused by ethanol as indicated in earlier study. In particular, RPS3 gene expression was increased by ethanol, glucosamine, and cytokines, implying that ethanol might decrease the metabolic activity by oxidative stress in β-cells. Therefore, cloning of these genes in full-length and the detailed studies of each gene on β-cell functions might provide clues on the pathophysiology of type 2 diabetes caused by alcohol.

Original languageEnglish
Pages (from-to)36-42
Number of pages7
JournalExperimental and Molecular Medicine
Volume36
Issue number1
Publication statusPublished - 2004 Feb 29

Fingerprint

Ethanol
Genes
Medical problems
Gene expression
Type 2 Diabetes Mellitus
Alcohols
Gene Expression
Signal transduction
Oxidative stress
Cloning
Glucosamine
Transcription
Cytoskeleton
Metabolism
Alcohol Drinking
Northern Blotting
Organism Cloning
Epidemiologic Studies
Signal Transduction
Oxidative Stress

Keywords

  • Differential expression
  • Ethanol
  • Pancreatic β-cell
  • RDA
  • Type 2 diabetes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Isolation of ethanol-induced genes in pancreatic β-cells by representational difference analysis (RDA). / Shin, Jun Seop; Kwon, Young Sam; Lee, Jae Jeong; Kim, Chan Wha.

In: Experimental and Molecular Medicine, Vol. 36, No. 1, 29.02.2004, p. 36-42.

Research output: Contribution to journalArticle

@article{6c71ac050465411ba487a780d520ca5f,
title = "Isolation of ethanol-induced genes in pancreatic β-cells by representational difference analysis (RDA)",
abstract = "Recent epidemiological studies suggest that alcohol consumption is one of the risk factors leading to type 2 diabetes, but the direct effect of ethanol on β-cell gene expression is not known. Here, using cDNA RDA method, we isolated 43 ethanol-induced genes in pancreatic β-cells, and confirmed their differential expression by Northern blot or semi-quantitative RT-PCR. These genes were further categorized by the functional criteria based on the published data; Translation, Transcription, Metabolism, Signal transduction, Transport, Structure, Cytoskeleton, Regulation, or Putative/Unknown genes. The effects of each gene on β-cell function need to be further investigated, however, the present data strongly suggest that these genes might be related to the metabolic alterations caused by ethanol as indicated in earlier study. In particular, RPS3 gene expression was increased by ethanol, glucosamine, and cytokines, implying that ethanol might decrease the metabolic activity by oxidative stress in β-cells. Therefore, cloning of these genes in full-length and the detailed studies of each gene on β-cell functions might provide clues on the pathophysiology of type 2 diabetes caused by alcohol.",
keywords = "Differential expression, Ethanol, Pancreatic β-cell, RDA, Type 2 diabetes",
author = "Shin, {Jun Seop} and Kwon, {Young Sam} and Lee, {Jae Jeong} and Kim, {Chan Wha}",
year = "2004",
month = "2",
day = "29",
language = "English",
volume = "36",
pages = "36--42",
journal = "Experimental and Molecular Medicine",
issn = "1226-3613",
publisher = "Korean Society of Med. Biochemistry and Mol. Biology",
number = "1",

}

TY - JOUR

T1 - Isolation of ethanol-induced genes in pancreatic β-cells by representational difference analysis (RDA)

AU - Shin, Jun Seop

AU - Kwon, Young Sam

AU - Lee, Jae Jeong

AU - Kim, Chan Wha

PY - 2004/2/29

Y1 - 2004/2/29

N2 - Recent epidemiological studies suggest that alcohol consumption is one of the risk factors leading to type 2 diabetes, but the direct effect of ethanol on β-cell gene expression is not known. Here, using cDNA RDA method, we isolated 43 ethanol-induced genes in pancreatic β-cells, and confirmed their differential expression by Northern blot or semi-quantitative RT-PCR. These genes were further categorized by the functional criteria based on the published data; Translation, Transcription, Metabolism, Signal transduction, Transport, Structure, Cytoskeleton, Regulation, or Putative/Unknown genes. The effects of each gene on β-cell function need to be further investigated, however, the present data strongly suggest that these genes might be related to the metabolic alterations caused by ethanol as indicated in earlier study. In particular, RPS3 gene expression was increased by ethanol, glucosamine, and cytokines, implying that ethanol might decrease the metabolic activity by oxidative stress in β-cells. Therefore, cloning of these genes in full-length and the detailed studies of each gene on β-cell functions might provide clues on the pathophysiology of type 2 diabetes caused by alcohol.

AB - Recent epidemiological studies suggest that alcohol consumption is one of the risk factors leading to type 2 diabetes, but the direct effect of ethanol on β-cell gene expression is not known. Here, using cDNA RDA method, we isolated 43 ethanol-induced genes in pancreatic β-cells, and confirmed their differential expression by Northern blot or semi-quantitative RT-PCR. These genes were further categorized by the functional criteria based on the published data; Translation, Transcription, Metabolism, Signal transduction, Transport, Structure, Cytoskeleton, Regulation, or Putative/Unknown genes. The effects of each gene on β-cell function need to be further investigated, however, the present data strongly suggest that these genes might be related to the metabolic alterations caused by ethanol as indicated in earlier study. In particular, RPS3 gene expression was increased by ethanol, glucosamine, and cytokines, implying that ethanol might decrease the metabolic activity by oxidative stress in β-cells. Therefore, cloning of these genes in full-length and the detailed studies of each gene on β-cell functions might provide clues on the pathophysiology of type 2 diabetes caused by alcohol.

KW - Differential expression

KW - Ethanol

KW - Pancreatic β-cell

KW - RDA

KW - Type 2 diabetes

UR - http://www.scopus.com/inward/record.url?scp=1642265739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642265739&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 36

EP - 42

JO - Experimental and Molecular Medicine

JF - Experimental and Molecular Medicine

SN - 1226-3613

IS - 1

ER -