Isolation of extracellular vesicles from small volumes of plasma using a microfluidic aqueous two-phase system

Bo Hoon Han, Sumi Kim, Geeyoon Seo, Youhee Heo, Seok Chung, Ji Yoon Kang

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

As conventional bulky methods for extracellular vesicle (EV) separation are unsuitable for small volumes of samples, microfluidic devices are thought to offer a solution for the integrated and automatic processing of EV separation. This study demonstrates a simple microfluidic aqueous two-phase system (ATPS) for EV separation with high recovery efficiency to overcome the limitation of previous devices, which require complex external equipment or high cost manufacturing. With polyethylene glycol and dextran in the microfluidic channel, the isolation mechanism of the microfluidic ATPS was analyzed by comparison between two-phase and one-phase systems. Our device could facilitate continuous EV isolation with 83.4% recovery efficiency and remove 65.4% of the proteins from the EV-protein mixture. EVs were also successfully isolated from human plasma at high recovery efficiency.

Original languageEnglish
Pages (from-to)3552-3559
Number of pages8
JournalLab on a Chip
Volume20
Issue number19
DOIs
Publication statusPublished - 2020 Oct 7

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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