Isolation of pepper mRNAs differentially expressed during the hypersensitive response to tobacco mosaic virus and characterization of a proteinase inhibitor gene

Ryoung Shin, Gil Je Lee, Chang Jin Park, Tae Yeon Kim, Jin Sam You, Young Woo Nam, Kyung Hee Paek

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Pepper (Capsicum annuum L. cv. Bugang) displays a hypersensitive response (HR) upon infection by tobacco mosaic virus (TMV) pathotype P0. To elucidate molecular mechanisms underlying this response, a cDNA library was constructed and differentially screened between TMV-P0-inoculated and uninoculated leaves. Of 3260 clones analyzed by reverse RNA slot-blot hybridization, 22 showed up-regulated expression upon TMV-P0 challenge. Among these, 16 clones showed significantly higher levels of expression in response to the avirulent pathotype TMV-P0 than to the virulent pathotype TMV-P1.2. Nucleotide sequence analysis and database searches revealed that the polypeptides deduced from the selected clones displayed significant sequence homology to various known proteins, indicating conservation of the wide range of up-regulated proteins during HR against viral challenge in higher plants. A cDNA clone encoding a putative proteinase inhibitor II (CaPinII) was selected and further analyzed. Upon TMV-P0 inoculation, the CaPinII transcript accumulated maximally at 72 h post-inoculation and declined afterwards. In contrast, inoculation with TMV-P1.2 did not induce CaPinII expression to any detectable level. The CaPinII gene was also expressed in response to the treatment of various chemicals, as well as wounding. Southern blot analysis showed that a small family of genes homologous to the CaPinII gene is present in the pepper genome.

Original languageEnglish
Pages (from-to)727-737
Number of pages11
JournalPlant Science
Volume161
Issue number4
DOIs
Publication statusPublished - 2001 Sep 26

Fingerprint

Tobacco Mosaic Virus
Tobacco mosaic virus
Tobacco
hypersensitive response
proteinase inhibitors
Viruses
pepper
Peptide Hydrolases
Clone Cells
Genes
clones
Messenger RNA
pathotypes
genes
Capsicum
Gene Library
chemical treatment
Sequence Homology
Capsicum annuum
Southern Blotting

Keywords

  • cDNA cloning
  • Differential screening
  • Hot pepper (Capsicum annuum L.)
  • Hypersensitive response
  • Proteinase inhibitor II
  • Tobacco mosaic virus

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Isolation of pepper mRNAs differentially expressed during the hypersensitive response to tobacco mosaic virus and characterization of a proteinase inhibitor gene. / Shin, Ryoung; Lee, Gil Je; Park, Chang Jin; Kim, Tae Yeon; You, Jin Sam; Nam, Young Woo; Paek, Kyung Hee.

In: Plant Science, Vol. 161, No. 4, 26.09.2001, p. 727-737.

Research output: Contribution to journalArticle

Shin, Ryoung ; Lee, Gil Je ; Park, Chang Jin ; Kim, Tae Yeon ; You, Jin Sam ; Nam, Young Woo ; Paek, Kyung Hee. / Isolation of pepper mRNAs differentially expressed during the hypersensitive response to tobacco mosaic virus and characterization of a proteinase inhibitor gene. In: Plant Science. 2001 ; Vol. 161, No. 4. pp. 727-737.
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