Isolation of side population cells in B-cell non-Hodgkin's lymphomas

Mi Ran Lee, Hyun Jeong Ju, Byung Soo Kim, Young Hyeh Ko, Won Seog Kim, Seok Jin Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Side population (SP) cells are characterized by the ability to exclude Hoechst 33342 dye due to high expression of the ATP-binding cassette transporter. This ability is associated with drug-resistant characteristics of cancer stem cells. Methods: We analyzed SP cells from human B-cell non-Hodgkin's lymphoma cell lines and primary cells derived from patients and compared them with non-SP (NSP) cells. Results: SP cells comprised a minor fraction of all cells ranging from 1.5 ± 1.8 to 8.3 ± 5.7% in cell lines and had higher ABCG2 expression than NSP cells. SP cells had better cell viability, colony-forming ability and drug resistance than NSP cells. The SP cells also showed stem cell-like characteristics, including elevated telomerase activity and higher expression of OCT4 and NANOG. A cDNA microarray demonstrated that SP cells had decreased expression of genes associated with apoptosis and cell death compared to NSP cells. Conclusions: The presence of SP cells might imply the possibility of lymphoma stem cells and be associated with a malignant potential of B-cell lymphoma.

Original languageEnglish
Pages (from-to)10-17
Number of pages8
JournalActa Haematologica
Volume129
Issue number1
DOIs
Publication statusPublished - 2013 Jan

Keywords

  • B-cell lymphoma
  • Cancer stem cells
  • Non-Hodgkin's lymphoma
  • Side population
  • Stem cell-like factors

ASJC Scopus subject areas

  • Hematology

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