Isolation of spheroid-forming single cells from gastric cancer cell lines: enrichment of cancer stem-like cells

Jong Won Lee, Jae Sook Sung, Young Soo Park, Seok Chung, Yeul Hong Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Isolation of spheroid-forming cells is important to investigate cancer stem cell (CSC) characteristics. However, conventional tumor spheroid culture methods have not proven suitable because the aggregated spheroids generally maintain their original heterogeneity and harbor multiple cells with various characteristics. Here we cultured spheroids using a polydimethylsiloxane microwell-based method and a limiting dilution protocol. We then isolated and enriched for CSCs that formed single cell-derived spheroids from gastric cancer cell lines. Cells from the microwell demonstrated higher self-renewal, increased expression of stem cell markers and resistance to apoptosis compared with spheroid cells made by the traditional method. This novel approach allows efficient cancer stem cell isolation and represents a step forward in cancer stem cell studies.

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalBioTechniques
Volume65
Issue number4
DOIs
Publication statusPublished - 2018 Oct 1

Keywords

  • cancer stem cells
  • gastric cancer
  • spheroids

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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