Isotype and IgG subclass distribution of autoantibody response to alpha-enolase protein in adult patients with severe Asthma

Hye Ah Lee, Byul Kwon, Gyu Young Hur, Sung Jin Choi, Dong Ho Nahm, Hae Sim Park

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Purpose: A possible involvement of autoimmune mechanism in the pathogenesis of bronchial asthma has been proposed. Recently, alpha-enolase protein was identified as a major autoantigen recognized by circulating IgG autoantibodies in patients with severe asthma. To evaluate a possible pathogenetic significance of these autoantibodies in severe asthma, isotype (IgG, IgA, IgM, and IgE) and IgG subclass (IgG1, IgG2, IgG3, and IgG4) distributions of autoantibodies to recombinant human alpha-enolase protein were analyzed. Patients and Methods: We examined serum samples from 10 patients with severe asthma and 7 patients with mild-to-moderate asthma, and 5 healthy controls by immunoblot analysis. Severe asthma was defined as patients having at least 1 severe asthmatic exacerbation requiring an emergency department visit or admission in the last year despite continuous typical therapies. Results: IgG1 was the predominant IgG subclass antibody response to alpha-enolase protein in patients with severe asthma. IgG1 autoantibody to alpha-enolase protein was detected in 7 of 10 patients with severe asthma (70%), 1 of 7 patients with mild-to-moderate asthma (14.3%), and none of 5 healthy controls (0%) (chi-square test; p < 0.05). IgA, IgM, and IgE autoantibodies to alpha-enolase protein could not be detected in patients with severe asthma. Conclusion: IgG1 subclass was the predominant type of autoantibody response to alpha-enolase protein in patients with severe asthma, suggests a possibility of IgG1 autoantibody-mediated complement activation in the pathogenesis of severe asthma.

    Original languageEnglish
    Pages (from-to)923-930
    Number of pages8
    JournalYonsei medical journal
    Volume49
    Issue number6
    DOIs
    Publication statusPublished - 2008

    Keywords

    • Asthma
    • Autoantibody
    • IgE
    • IgG
    • IgG subclass

    ASJC Scopus subject areas

    • Medicine(all)

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