TY - JOUR
T1 - Istamycin aminoglycosides profiling and their characterization in Streptomyces tenjimariensis ATCC 31603 culture using high-performance liquid chromatography with tandem mass spectrometry
AU - Hoang, Nguyen Huu
AU - Huong, Nguyen Lan
AU - Kim, Byul
AU - Sohng, Jae Kyung
AU - Yoon, Yeo Joon
AU - Park, Je Won
N1 - Funding Information:
The authors acknowledge the financial supports by the National Research Foundation of Korea grant (2015R1A2A2A01002524, 2016R1A2A1A05005078 [Y.J.Y]) funded by the Ministry of Science, ICT and Future Planning, and by the grant (PJ011066) funded by the Next-Generation BioGreen21 program, Rural Development Administration.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - A high-performance liquid chromatography with electrospray ionization ion trap tandem mass spectrometry method was developed and validated for the robust profiling and characterization of biosynthetic congeners in the 2-deoxy-aminocyclitol istamycin pathway, from the fermentation broth of Streptomyces tenjimariensis ATCC 31603. Gradient elution on an Acquity CSH C18 column was performed with a gradient of 5 mM aqueous pentafluoropropionic acid and 50% acetonitrile. Sixteen natural istamycin congeners were profiled and quantified in descending order; istamycin A, istamycin B, istamycin A0, istamycin B0, istamycin B1, istamycin A1, istamycin C, istamycin A2, istamycin C1, istamycin C0, istamycin X0, istamycin A3, istamycin Y0, istamycin B3, and istamycin FU-10 plus istamycin AP. In addition, a total of five sets of 1- or 3-epimeric pairs were chromatographically separated using a macrocyclic glycopeptide-bonded chiral column. The lower limit of quantification of istamycin-A present in S. tenjimariensis fermentation was estimated to be 2.2 ng/mL. The simultaneous identification of a wide range of 2-deoxy-aminocyclitol-type istamycin profiles from bacterial fermentation was determined for the first time by employing high-performance liquid chromatography with tandem mass spectrometry analysis and the separation of istamycin epimers.
AB - A high-performance liquid chromatography with electrospray ionization ion trap tandem mass spectrometry method was developed and validated for the robust profiling and characterization of biosynthetic congeners in the 2-deoxy-aminocyclitol istamycin pathway, from the fermentation broth of Streptomyces tenjimariensis ATCC 31603. Gradient elution on an Acquity CSH C18 column was performed with a gradient of 5 mM aqueous pentafluoropropionic acid and 50% acetonitrile. Sixteen natural istamycin congeners were profiled and quantified in descending order; istamycin A, istamycin B, istamycin A0, istamycin B0, istamycin B1, istamycin A1, istamycin C, istamycin A2, istamycin C1, istamycin C0, istamycin X0, istamycin A3, istamycin Y0, istamycin B3, and istamycin FU-10 plus istamycin AP. In addition, a total of five sets of 1- or 3-epimeric pairs were chromatographically separated using a macrocyclic glycopeptide-bonded chiral column. The lower limit of quantification of istamycin-A present in S. tenjimariensis fermentation was estimated to be 2.2 ng/mL. The simultaneous identification of a wide range of 2-deoxy-aminocyclitol-type istamycin profiles from bacterial fermentation was determined for the first time by employing high-performance liquid chromatography with tandem mass spectrometry analysis and the separation of istamycin epimers.
KW - Chiral columns
KW - Epimeric mixtures
KW - Istamycin aminoglycoside
KW - Streptomyces tenjimariensis
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U2 - 10.1002/jssc.201600925
DO - 10.1002/jssc.201600925
M3 - Article
C2 - 27778478
AN - SCOPUS:85005996224
SN - 1615-9306
VL - 39
SP - 4712
EP - 4722
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 24
ER -