JAB1 regulates CPNE1-related differentiation via direct binding to CPNE1 in HiB5 hippocampal progenitor cells

Jae Cheal Yoo, Nammi Park, Hye Young Choi, Jae-Yong Park, Gwan Su Yi

Research output: Contribution to journalArticle

Abstract

Copine1 (CPNE1), has tandem C2 domains and an A domain. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Protein kinase B (AKT) phosphorylation in the hippocampal progenitor cell line, HiB5. To better understand its cellular function, we carried out a yeast two-hybrid screening to find CPNE1 binding partners. Among the identified proteins, Jun activation domain-binding protein 1 (JAB1) appears to directly interact with CPNE1. Between CPNE1 and JAB1, the physical interaction was confirmed in vitro and in vivo. In addition the specific binding regions of CPNE1 and JAB1 was confirmed with truncated mutant assay. Furthermore, our results also demonstrate that AKT phosphorylation and expression of the neuronal marker protein are increased when JAB1 is overexpressed in CPNE1 high expressed HiB5 cells. Moreover, overexpression of both CPNE1 and JAB1 in HiB5 cells effectively increased neurite outgrowth. Collectively, our findings suggest that JAB1 activates the neuronal differentiation ability of CPNE1 through the binding of C2A domain in CPNE1 with MPN domain in JAB1.

Original languageEnglish
Pages (from-to)424-429
Number of pages6
JournalBiochemical and biophysical research communications
Volume497
Issue number1
DOIs
Publication statusPublished - 2018 Feb 26

Fingerprint

Carrier Proteins
Stem Cells
Chemical activation
Phosphorylation
Proto-Oncogene Proteins c-akt
Yeast
Assays
Screening
Proteins
Yeasts
Cells
Cell Line

Keywords

  • C2A domain
  • Copine1
  • JAB1
  • MPN domain
  • Protein-protein interaction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

JAB1 regulates CPNE1-related differentiation via direct binding to CPNE1 in HiB5 hippocampal progenitor cells. / Yoo, Jae Cheal; Park, Nammi; Choi, Hye Young; Park, Jae-Yong; Yi, Gwan Su.

In: Biochemical and biophysical research communications, Vol. 497, No. 1, 26.02.2018, p. 424-429.

Research output: Contribution to journalArticle

@article{f38708dcc25d493887e571ae93effab0,
title = "JAB1 regulates CPNE1-related differentiation via direct binding to CPNE1 in HiB5 hippocampal progenitor cells",
abstract = "Copine1 (CPNE1), has tandem C2 domains and an A domain. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Protein kinase B (AKT) phosphorylation in the hippocampal progenitor cell line, HiB5. To better understand its cellular function, we carried out a yeast two-hybrid screening to find CPNE1 binding partners. Among the identified proteins, Jun activation domain-binding protein 1 (JAB1) appears to directly interact with CPNE1. Between CPNE1 and JAB1, the physical interaction was confirmed in vitro and in vivo. In addition the specific binding regions of CPNE1 and JAB1 was confirmed with truncated mutant assay. Furthermore, our results also demonstrate that AKT phosphorylation and expression of the neuronal marker protein are increased when JAB1 is overexpressed in CPNE1 high expressed HiB5 cells. Moreover, overexpression of both CPNE1 and JAB1 in HiB5 cells effectively increased neurite outgrowth. Collectively, our findings suggest that JAB1 activates the neuronal differentiation ability of CPNE1 through the binding of C2A domain in CPNE1 with MPN domain in JAB1.",
keywords = "C2A domain, Copine1, JAB1, MPN domain, Protein-protein interaction",
author = "Yoo, {Jae Cheal} and Nammi Park and Choi, {Hye Young} and Jae-Yong Park and Yi, {Gwan Su}",
year = "2018",
month = "2",
day = "26",
doi = "10.1016/j.bbrc.2018.02.101",
language = "English",
volume = "497",
pages = "424--429",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - JAB1 regulates CPNE1-related differentiation via direct binding to CPNE1 in HiB5 hippocampal progenitor cells

AU - Yoo, Jae Cheal

AU - Park, Nammi

AU - Choi, Hye Young

AU - Park, Jae-Yong

AU - Yi, Gwan Su

PY - 2018/2/26

Y1 - 2018/2/26

N2 - Copine1 (CPNE1), has tandem C2 domains and an A domain. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Protein kinase B (AKT) phosphorylation in the hippocampal progenitor cell line, HiB5. To better understand its cellular function, we carried out a yeast two-hybrid screening to find CPNE1 binding partners. Among the identified proteins, Jun activation domain-binding protein 1 (JAB1) appears to directly interact with CPNE1. Between CPNE1 and JAB1, the physical interaction was confirmed in vitro and in vivo. In addition the specific binding regions of CPNE1 and JAB1 was confirmed with truncated mutant assay. Furthermore, our results also demonstrate that AKT phosphorylation and expression of the neuronal marker protein are increased when JAB1 is overexpressed in CPNE1 high expressed HiB5 cells. Moreover, overexpression of both CPNE1 and JAB1 in HiB5 cells effectively increased neurite outgrowth. Collectively, our findings suggest that JAB1 activates the neuronal differentiation ability of CPNE1 through the binding of C2A domain in CPNE1 with MPN domain in JAB1.

AB - Copine1 (CPNE1), has tandem C2 domains and an A domain. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Protein kinase B (AKT) phosphorylation in the hippocampal progenitor cell line, HiB5. To better understand its cellular function, we carried out a yeast two-hybrid screening to find CPNE1 binding partners. Among the identified proteins, Jun activation domain-binding protein 1 (JAB1) appears to directly interact with CPNE1. Between CPNE1 and JAB1, the physical interaction was confirmed in vitro and in vivo. In addition the specific binding regions of CPNE1 and JAB1 was confirmed with truncated mutant assay. Furthermore, our results also demonstrate that AKT phosphorylation and expression of the neuronal marker protein are increased when JAB1 is overexpressed in CPNE1 high expressed HiB5 cells. Moreover, overexpression of both CPNE1 and JAB1 in HiB5 cells effectively increased neurite outgrowth. Collectively, our findings suggest that JAB1 activates the neuronal differentiation ability of CPNE1 through the binding of C2A domain in CPNE1 with MPN domain in JAB1.

KW - C2A domain

KW - Copine1

KW - JAB1

KW - MPN domain

KW - Protein-protein interaction

UR - http://www.scopus.com/inward/record.url?scp=85042044308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042044308&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2018.02.101

DO - 10.1016/j.bbrc.2018.02.101

M3 - Article

C2 - 29448099

AN - SCOPUS:85042044308

VL - 497

SP - 424

EP - 429

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -