JIP1 binding to RBP-Jk mediates cross-talk between the Notch1 and JIP1-JNK signaling pathway

M. Y. Kim, E. J. Ann, J. S. Mo, F. Dajas-Bailador, M. S. Seo, J. A. Hong, J. Jung, Y. H. Choi, J. H. Yoon, S. M. Kim, Eui Ju Choi, H. S. Hoe, A. J. Whitmarsh, H. S. Park

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Notch1 signaling has a critical function in maintaining a balance among cell proliferation, differentiation, and apoptosis. Our earlier work showed that the Notch1 intracellular domain interferes with the scaffolding function of c-Jun N-terminal kinase (JNK)-interacting protein-1 (JIP1), yet the effect of JIP1 for Notch1-recombining binding protein suppressor of hairless (RBP-Jk) signaling remains unknown. Here, we show that JIP1 suppresses Notch1 activity. JIP1 was found to physically associate with either intracellular domain of Notch1 or RBP-Jk and interfere with the interaction between them. Furthermore, we ascertained that JIP1 caused the cytoplasmic retention of RBP-Jk through an interaction between the C-terminal region of JIP1 including Src homology 3 domain and the proline-rich domain of RBP-Jk. We also found that RBP-Jk inhibits JIP1-mediated activation of the JNK1 signaling cascade and cell death. Our results suggest that direct protein-protein interactions coordinate cross-talk between the Notch1-RBP-Jk and JIP1-JNK pathways.

Original languageEnglish
Pages (from-to)1728-1738
Number of pages11
JournalCell Death and Differentiation
Volume17
Issue number11
DOIs
Publication statusPublished - 2010 Nov 1

Fingerprint

Protein Binding
Phosphotransferases
Proteins
Notch1 Receptor
src Homology Domains
JNK Mitogen-Activated Protein Kinases
Proline
Cell Differentiation
Carrier Proteins
Cell Death
Cell Proliferation
Apoptosis

Keywords

  • cytoplasmic retention
  • JIP1
  • Notch1
  • RBP-Jk
  • SH3 domain

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Kim, M. Y., Ann, E. J., Mo, J. S., Dajas-Bailador, F., Seo, M. S., Hong, J. A., ... Park, H. S. (2010). JIP1 binding to RBP-Jk mediates cross-talk between the Notch1 and JIP1-JNK signaling pathway. Cell Death and Differentiation, 17(11), 1728-1738. https://doi.org/10.1038/cdd.2010.50

JIP1 binding to RBP-Jk mediates cross-talk between the Notch1 and JIP1-JNK signaling pathway. / Kim, M. Y.; Ann, E. J.; Mo, J. S.; Dajas-Bailador, F.; Seo, M. S.; Hong, J. A.; Jung, J.; Choi, Y. H.; Yoon, J. H.; Kim, S. M.; Choi, Eui Ju; Hoe, H. S.; Whitmarsh, A. J.; Park, H. S.

In: Cell Death and Differentiation, Vol. 17, No. 11, 01.11.2010, p. 1728-1738.

Research output: Contribution to journalArticle

Kim, MY, Ann, EJ, Mo, JS, Dajas-Bailador, F, Seo, MS, Hong, JA, Jung, J, Choi, YH, Yoon, JH, Kim, SM, Choi, EJ, Hoe, HS, Whitmarsh, AJ & Park, HS 2010, 'JIP1 binding to RBP-Jk mediates cross-talk between the Notch1 and JIP1-JNK signaling pathway', Cell Death and Differentiation, vol. 17, no. 11, pp. 1728-1738. https://doi.org/10.1038/cdd.2010.50
Kim, M. Y. ; Ann, E. J. ; Mo, J. S. ; Dajas-Bailador, F. ; Seo, M. S. ; Hong, J. A. ; Jung, J. ; Choi, Y. H. ; Yoon, J. H. ; Kim, S. M. ; Choi, Eui Ju ; Hoe, H. S. ; Whitmarsh, A. J. ; Park, H. S. / JIP1 binding to RBP-Jk mediates cross-talk between the Notch1 and JIP1-JNK signaling pathway. In: Cell Death and Differentiation. 2010 ; Vol. 17, No. 11. pp. 1728-1738.
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abstract = "Notch1 signaling has a critical function in maintaining a balance among cell proliferation, differentiation, and apoptosis. Our earlier work showed that the Notch1 intracellular domain interferes with the scaffolding function of c-Jun N-terminal kinase (JNK)-interacting protein-1 (JIP1), yet the effect of JIP1 for Notch1-recombining binding protein suppressor of hairless (RBP-Jk) signaling remains unknown. Here, we show that JIP1 suppresses Notch1 activity. JIP1 was found to physically associate with either intracellular domain of Notch1 or RBP-Jk and interfere with the interaction between them. Furthermore, we ascertained that JIP1 caused the cytoplasmic retention of RBP-Jk through an interaction between the C-terminal region of JIP1 including Src homology 3 domain and the proline-rich domain of RBP-Jk. We also found that RBP-Jk inhibits JIP1-mediated activation of the JNK1 signaling cascade and cell death. Our results suggest that direct protein-protein interactions coordinate cross-talk between the Notch1-RBP-Jk and JIP1-JNK pathways.",
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