JNK activation induced by ribotoxic stress is initiated from 80S monosomes but not polysomes

Tae Sung Kim, Hag Dong Kim, Yong Jun Park, Eun Bin Kong, Hee Woong Yang, Youjin Jung, Yong Joong Kim, Joon Kim

Research output: Contribution to journalArticle

Abstract

Translation is a costly, but inevitable, cell maintenance process. To reduce unnecessary ATP consumption in cells, a fine-tuning mechanism is needed for both ribosome biogenesis and translation. Previous studies have suggested that the ribosome functions as a hub for many cellular signals such as ribotoxic stress response, mammalian target of rapamycin (mTOR), and ribosomal S6 kinase (RSK) signaling. Therefore, we investigated the relationship between ribosomes and mitogen-activated protein kinase (MAPK) activation under ribotoxic stress conditions and found that the activation of c-Jun N-terminal kinases (JNKs) was suppressed by ribosomal protein knockdown but that of p38 was not. In addition, we found that JNK activation is driven by the association of inactive JNK in the 80S monosomes rather than the polysomes. Overall, these data suggest that the activation of JNKs by ribotoxic stress is attributable to 80S monosomes. These 80S monosomes are active ribosomes that are ready to initiate protein translation, rather than polysomes that are already acting ribosomes involved in translation elongation.

Original languageEnglish
Pages (from-to)502-507
Number of pages6
JournalBMB reports
Volume52
Issue number8
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Polyribosomes
Ribosomes
Phosphotransferases
Chemical activation
Ribosomal Protein S6 Kinases
JNK Mitogen-Activated Protein Kinases
Ribosomal Proteins
Sirolimus
Mitogen-Activated Protein Kinases
Protein Biosynthesis
Elongation
Tuning
Adenosine Triphosphate
Association reactions
Maintenance
Proteins

Keywords

  • 80S monosome
  • Deoxynivalenol
  • Emetine
  • JNK
  • Ribotoxic stress

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

JNK activation induced by ribotoxic stress is initiated from 80S monosomes but not polysomes. / Kim, Tae Sung; Kim, Hag Dong; Park, Yong Jun; Kong, Eun Bin; Yang, Hee Woong; Jung, Youjin; Kim, Yong Joong; Kim, Joon.

In: BMB reports, Vol. 52, No. 8, 01.01.2019, p. 502-507.

Research output: Contribution to journalArticle

Kim, TS, Kim, HD, Park, YJ, Kong, EB, Yang, HW, Jung, Y, Kim, YJ & Kim, J 2019, 'JNK activation induced by ribotoxic stress is initiated from 80S monosomes but not polysomes', BMB reports, vol. 52, no. 8, pp. 502-507. https://doi.org/10.5483/BMBRep.2019.52.8.273
Kim, Tae Sung ; Kim, Hag Dong ; Park, Yong Jun ; Kong, Eun Bin ; Yang, Hee Woong ; Jung, Youjin ; Kim, Yong Joong ; Kim, Joon. / JNK activation induced by ribotoxic stress is initiated from 80S monosomes but not polysomes. In: BMB reports. 2019 ; Vol. 52, No. 8. pp. 502-507.
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AU - Jung, Youjin

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AU - Kim, Joon

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