Kainate treatment alters TGF-β3 gene expression in the rat hippocampus

Hyoung Chun Kim, Guoying Bing, Seong J. Kim, Wang K. Jhoo, Eun J. Shin, Myung Bok Wie, Kwang H. Ko, Won-Ki Kim, Kathleen C. Flanders, Shin Geon Choi, Jau Shyong Hong

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11 Citations (Scopus)


In order to evaluate the role of transforming growth factor (TGF)-β3 in the neurodegenerative process, we examined the levels of mRNA and immunocytochemical distribution for TGF-β3 in the rat hippocampus after systemic kainic acid (KA) administration. Hippocampal TGF-β3 mRNA level was reduced 3 h after KA injection. However, the levels of TGF-β3 mRNA were elevated 1 day post-KA and lasted for at least 30 days. A mild TGF-β3 immunoreactivity (TGF-β3-IR) in the Ammon's horn and a moderate TGF-β3-IR in the dentate granule cells were observed in the normal hippocampus. The CA1 and CA3 neurons lost their TGF-β3-IR, while TGF-β3-positive glia-like cells proliferated mainly throughout the CA1 sector and had an intense immunoreactivity at 7, 15 and 30 days after KA. This immunocytochemical distribution of TGF-β3-positive non-neuronal populations was similar to that of glial fibrillary acidic protein (GFAP)-positive cells. Double labeling immunocytochemical analysis demonstrated colocalization of TGF-β3- and GFAP-immunoreactivity in the same cells. These findings suggest a compensatory mechanism of astrocytes for the synthesis of TGF-β3 protein in response to KA-induced neurodegeneration. In addition, exogenous TGF-β3 (5 or 10 ng/i.c.v.) significantly attenuated KA-induced seizures and neuronal damages in a dose-related manner. Therefore, our results suggest that TGF-β3 plays an important role in protective mechanisms against KA-induced neurodegeneration.

Original languageEnglish
Pages (from-to)60-70
Number of pages11
JournalMolecular Brain Research
Issue number1-2
Publication statusPublished - 2002 Dec 16
Externally publishedYes


  • Astrocyte
  • Exogenous TGF-β3
  • Hippocampus
  • Kainic acid
  • Neurodegeneration
  • Transforming growth factor-β3

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Kim, H. C., Bing, G., Kim, S. J., Jhoo, W. K., Shin, E. J., Wie, M. B., Ko, K. H., Kim, W-K., Flanders, K. C., Choi, S. G., & Hong, J. S. (2002). Kainate treatment alters TGF-β3 gene expression in the rat hippocampus. Molecular Brain Research, 108(1-2), 60-70. https://doi.org/10.1016/S0169-328X(02)00514-4