Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 RTA reactivates murine gammaherpesvirus 68 from latency

Tammy M. Rickabaugh, Helen J. Brown, Ting Ting Wu, Moon Jung Song, Seungmin Hwang, Hongyu Deng, Katherine Mitsouras, Ren Sun

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Murine gammaherpesvirus 68 (MHV-68), Kaposi's sarcoma-associated herpesvirus (HHV-8), and Epsiein-Barr virus (EBV) are all members of the gammaherpesvirus family, characterized by their ability to establish latency in lymphocytes. The RTA protein, conserved in all gammaherpesvinises, is known to play a critical role in reactivation from latency. Here we report that HHV-8 RTA, not EBV RTA, was able to induce MHV-68 lytic viral proteins and DNA replication and processing and produce viable MHV-68 virions from latently infected cells at levels similar to those for MHV-68 RTA. HHV-8 RTA was also able to activate two MHV-68 lytic promoters, whereas EBV RTA was not. In order to define the domains of RTA responsible for their functional differences in viral promoter activation and initiation of the MHV-68 lytic cycle, chimeric RTA proteins were constructed by exchanging the N-terminal and C-terminal domains of the RTA proteins. Our data suggest that the species specificity of MHV-68 RTA resides in the N-terminal DNA binding domain.

Original languageEnglish
Pages (from-to)3217-3222
Number of pages6
JournalJournal of Virology
Volume79
Issue number5
DOIs
Publication statusPublished - 2005 Mar 1
Externally publishedYes

Fingerprint

Human Herpesvirus 8
Viruses
Species Specificity
Virus Activation
Aptitude
Viral DNA
Viral Proteins
DNA Replication
Virion
Proteins
Lymphocytes
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 RTA reactivates murine gammaherpesvirus 68 from latency. / Rickabaugh, Tammy M.; Brown, Helen J.; Wu, Ting Ting; Song, Moon Jung; Hwang, Seungmin; Deng, Hongyu; Mitsouras, Katherine; Sun, Ren.

In: Journal of Virology, Vol. 79, No. 5, 01.03.2005, p. 3217-3222.

Research output: Contribution to journalArticle

Rickabaugh, Tammy M. ; Brown, Helen J. ; Wu, Ting Ting ; Song, Moon Jung ; Hwang, Seungmin ; Deng, Hongyu ; Mitsouras, Katherine ; Sun, Ren. / Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 RTA reactivates murine gammaherpesvirus 68 from latency. In: Journal of Virology. 2005 ; Vol. 79, No. 5. pp. 3217-3222.
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