Kappa-carrageenan-based dual crosslinkable bioink for extrusion type bioprinting

Wonseop Lim, Gyeong Jin Kim, Hyun Woo Kim, Jiyeon Lee, Xiaowei Zhang, Min Gyeong Kang, Jeong Wook Seo, Jae Min Cha, Hyun Jin Park, Min Young Lee, Su Ryon Shin, Seon Young Shin, Hojae Bae

Research output: Contribution to journalArticlepeer-review

Abstract

Bioink based 3D bioprinting is a promising new technology that enables fabrication of complex tissue structures with living cells. The printability of the bioink depends on the physical properties such as viscosity. However, the high viscosity bioink puts shear stress on the cells and low viscosity bioink cannot maintain complex tissue structure firmly after the printing. In this work, we applied dual crosslinkable bioink using Kappa-carrageenan (κ-CA) to overcome existing shortcomings. κ-CA has properties such as biocompatibility, biodegradability, shear-thinning and ionic gelation but the difficulty of controlling gelation properties makes it unsuitable for application in 3D bioprinting. This problem was solved by synthesizing methacrylated Kappa-carrageenan (MA-κ-CA), which can be dual crosslinked through ionic and UV (Ultraviolet) crosslinking to form hydrogel using NIH-3T3 cells. Through MA substitutions, the rheological properties of the gel could be controlled to reduce the shear stress. Moreover, bioprinting using the cell-laden MA-κ-CA showed cell compatibility with enhanced shape retention capability. The potential to control the physical properties through dual crosslinking of MA-κ-CA hydrogel is expected to be widely applied in 3D bioprinting applications.

Original languageEnglish
Article number2377
Pages (from-to)1-15
Number of pages15
JournalPolymers
Volume12
Issue number10
DOIs
Publication statusPublished - 2020 Oct

Keywords

  • 3D bioprinting
  • Dual crosslinking hydrogel
  • Kappa-carrageenan
  • Methacrylic anhydride

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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