Kdm3b haploinsufficiency impairs the consolidation of cerebellum-dependent motor memory in mice

Yong Gyu Kim, Myeong Seong Bak, Ahbin Kim, Yujin Kim, Yun Cheol Chae, Ye Lee Kim, Yang Sook Chun, Joon Yong An, Sang Beom Seo, Sang Jeong Kim, Yong Seok Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Histone modifications are a key mechanism underlying the epigenetic regulation of gene expression, which is critically involved in the consolidation of multiple forms of memory. However, the roles of histone modifications in cerebellum-dependent motor learning and memory are not well understood. To test whether changes in histone methylation are involved in cerebellar learning, we used heterozygous Kdm3b knockout (Kdm3b+/−) mice, which show reduced lysine 9 on histone 3 (H3K9) demethylase activity. H3K9 di-methylation is significantly increased selectively in the granule cell layer of the cerebellum of Kdm3b+/− mice. In the cerebellum-dependent optokinetic response (OKR) learning, Kdm3b+/− mice show deficits in memory consolidation, whereas they are normal in basal oculomotor performance and OKR acquisition. In addition, RNA-seq analyses revealed that the expression levels of several plasticity-related genes were altered in the mutant cerebellum. Our study suggests that active regulation of histone methylation is critical for the consolidation of cerebellar motor memory.

Original languageEnglish
Article number106
JournalMolecular brain
Volume14
Issue number1
DOIs
Publication statusPublished - 2021 Dec

Keywords

  • Cerebellum
  • Histone modification
  • Kdm3b
  • Optokinetic response (OKR)

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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