Abstract
Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (β2m), glutathione S-transferase alpha (GST-a), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegalyand tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats withhistopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.
| Original language | English |
|---|---|
| Pages (from-to) | 179-185 |
| Number of pages | 7 |
| Journal | Toxicological Research |
| Volume | 28 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2012 Dec 1 |
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Keywords
- Cystatin C
- Kidney damage biomarkers
- Kidney injury molecule 1
- Kidney toxicity
- Paecilomyces sinclairiis
ASJC Scopus subject areas
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Kidney toxicity induced by 13 weeks exposure to the fruiting body of Paecilomyces sinclairii in rats. / Jeong, Mihye; Kim, Young Won; Min, Jeong Ran; Kwon, Min; Han, Beom Suk; Kim, Jeong-Gyu; Jeong, Sang Hee.
In: Toxicological Research, Vol. 28, No. 3, 01.12.2012, p. 179-185.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Kidney toxicity induced by 13 weeks exposure to the fruiting body of Paecilomyces sinclairii in rats
AU - Jeong, Mihye
AU - Kim, Young Won
AU - Min, Jeong Ran
AU - Kwon, Min
AU - Han, Beom Suk
AU - Kim, Jeong-Gyu
AU - Jeong, Sang Hee
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (β2m), glutathione S-transferase alpha (GST-a), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegalyand tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats withhistopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.
AB - Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (β2m), glutathione S-transferase alpha (GST-a), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegalyand tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats withhistopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.
KW - Cystatin C
KW - Kidney damage biomarkers
KW - Kidney injury molecule 1
KW - Kidney toxicity
KW - Paecilomyces sinclairiis
UR - http://www.scopus.com/inward/record.url?scp=84872556124&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872556124&partnerID=8YFLogxK
U2 - 10.5487/TR.2012.28.3.179
DO - 10.5487/TR.2012.28.3.179
M3 - Article
VL - 28
SP - 179
EP - 185
JO - Toxicological Research
JF - Toxicological Research
SN - 1976-8257
IS - 3
ER -