TY - JOUR
T1 - Kinase gene fusions in defined subsets of melanoma
AU - Turner, Jacqueline
AU - Couts, Kasey
AU - Sheren, Jamie
AU - Saichaemchan, Siriwimon
AU - Ariyawutyakorn, Witthawat
AU - Avolio, Izabela
AU - Cabral, Ethan
AU - Glogowska, Magdelena
AU - Amato, Carol
AU - Robinson, Steven
AU - Hintzsche, Jennifer
AU - Applegate, Allison
AU - Seelenfreund, Eric
AU - Gonzalez, Rita
AU - Wells, Keith
AU - Bagby, Stacey
AU - Tentler, John
AU - Tan, Aik Choon
AU - Wisell, Joshua
AU - Varella-Garcia, Marileila
AU - Robinson, William
N1 - Funding Information:
We wish to acknowledge the UCD Research Histology Shared Resource. We thank Dr. Theresa A. Boyle and Kim E. Ellison for assisting with immunohistochemistry.
Publisher Copyright:
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Genomic rearrangements resulting in activating kinase fusions have been increasingly described in a number of cancers including malignant melanoma, but their frequency in specific melanoma subtypes has not been reported. We used break-apart fluorescence in situ hybridization (FISH) to identify genomic rearrangements in tissues from 59 patients with various types of malignant melanoma including acral lentiginous, mucosal, superficial spreading, and nodular. We identified four genomic rearrangements involving the genes BRAF, RET, and ROS1. Of these, three were confirmed by Immunohistochemistry (IHC) or sequencing and one was found to be an ARMC10-BRAF fusion that has not been previously reported in melanoma. These fusions occurred in different subtypes of melanoma but all in tumors lacking known driver mutations. Our data suggest gene fusions are more common than previously thought and should be further explored particularly in melanomas lacking known driver mutations.
AB - Genomic rearrangements resulting in activating kinase fusions have been increasingly described in a number of cancers including malignant melanoma, but their frequency in specific melanoma subtypes has not been reported. We used break-apart fluorescence in situ hybridization (FISH) to identify genomic rearrangements in tissues from 59 patients with various types of malignant melanoma including acral lentiginous, mucosal, superficial spreading, and nodular. We identified four genomic rearrangements involving the genes BRAF, RET, and ROS1. Of these, three were confirmed by Immunohistochemistry (IHC) or sequencing and one was found to be an ARMC10-BRAF fusion that has not been previously reported in melanoma. These fusions occurred in different subtypes of melanoma but all in tumors lacking known driver mutations. Our data suggest gene fusions are more common than previously thought and should be further explored particularly in melanomas lacking known driver mutations.
KW - acral
KW - kinase
KW - melanoma
KW - pan-negative
KW - rearrangement
UR - http://www.scopus.com/inward/record.url?scp=85010899632&partnerID=8YFLogxK
U2 - 10.1111/pcmr.12560
DO - 10.1111/pcmr.12560
M3 - Article
C2 - 27864876
AN - SCOPUS:85010899632
SN - 1755-1471
VL - 30
SP - 53
EP - 62
JO - Pigment Cell and Melanoma Research
JF - Pigment Cell and Melanoma Research
IS - 1
ER -
