Kinetic analysis of RNA interference for lamin A/C in HeLa cells

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug-response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.

Original languageEnglish
Pages (from-to)623-627
Number of pages5
JournalActa Biochimica et Biophysica Sinica
Volume42
Issue number9
DOIs
Publication statusPublished - 2010 Sep 1

Fingerprint

Lamin Type A
RNA Interference
HeLa Cells
Small Interfering RNA
RNA
Kinetics
Gene Silencing
Genes
Nanostructures
Nanostructured materials
Gene expression
Cell Division
Dilution
Cells
Guidelines
Gene Expression
Recovery
Pharmaceutical Preparations

Keywords

  • gene silencing
  • kinetic analysis
  • lamin A/C
  • RNAi
  • siRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Kinetic analysis of RNA interference for lamin A/C in HeLa cells. / You, Sung Ju; Lee, Sang Hoon; Lee, Jae-Seung.

In: Acta Biochimica et Biophysica Sinica, Vol. 42, No. 9, 01.09.2010, p. 623-627.

Research output: Contribution to journalArticle

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