Kinetic analysis of RNA interference for lamin A/C in HeLa cells

Sung Ju You, Sang Hoon Lee, Jae Seung Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug-response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.

Original languageEnglish
Pages (from-to)623-627
Number of pages5
JournalActa Biochimica et Biophysica Sinica
Volume42
Issue number9
DOIs
Publication statusPublished - 2010 Sep

Keywords

  • RNAi
  • gene silencing
  • kinetic analysis
  • lamin A/C
  • siRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Fingerprint Dive into the research topics of 'Kinetic analysis of RNA interference for lamin A/C in HeLa cells'. Together they form a unique fingerprint.

  • Cite this