Kinetic limitations of a bioelectrochemical electrode using carbon nanotube-attached glucose oxidase for biofuel cells

Xueyan Zhao, Hongfei Jia, Jungbae Kim, Ping Wang

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Carbon nanotubes (CNTs) have been used for various bioelectrochemical applications, presumably for substantial improvement in performance. However, often only moderate results observed, with many governing factors have been considered and suggested yet without much systematic evaluation and verification. In this study, CNT-supported glucose oxidase (CNT-GOx) was examined in the presence of 1,4-benzoquinone (BQ). The intrinsic Michaelis parameters of the reaction catalyzed by CNT-GOx were found very close to those of native GOx. However, the Nafion entrapment of CNT-GOx for an electrode resulted in a much lower activity due to the limited availability of the embedded enzyme. Interestingly, kinetic studies revealed that the biofuel cell employing such an enzyme electrode only generated a power density equivalent to <40% of the reaction capability of the enzyme on electrode. It appeared to us that factors such as electron and proton transfer resistances can be more overwhelming than the heterogeneous reaction kinetics in limiting the power generation of such biofuel cells.

Original languageEnglish
Pages (from-to)1068-1074
Number of pages7
JournalBiotechnology and Bioengineering
Volume104
Issue number6
DOIs
Publication statusPublished - 2009 Dec 15

Fingerprint

Bioelectric Energy Sources
Biological fuel cells
Glucose Oxidase
Carbon Nanotubes
Glucose oxidase
Carbon nanotubes
Electrodes
Kinetics
Biosensing Techniques
Enzymes
Enzyme electrodes
Enzyme kinetics
Proton transfer
Reaction kinetics
Power generation
Protons
Availability
Electrons

Keywords

  • Biocatalysis
  • Biofuel cell
  • Biotransformation kinetics
  • Carbon nanotube
  • Enzyme electrode
  • Glucose oxidase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Kinetic limitations of a bioelectrochemical electrode using carbon nanotube-attached glucose oxidase for biofuel cells. / Zhao, Xueyan; Jia, Hongfei; Kim, Jungbae; Wang, Ping.

In: Biotechnology and Bioengineering, Vol. 104, No. 6, 15.12.2009, p. 1068-1074.

Research output: Contribution to journalArticle

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