Kinetic modeling of simultaneous saccharification and fermentation for ethanol production using steam-exploded wood with glucose- and cellobiose-fermenting yeast, Brettanomyces custersii

H. Moon, J. S. Kim, K. K. Oh, Seung Wook Kim, S. I. Hong

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A mathematical model is proposed that can depict the kinetics of simultaneous saccharification and fermentation (SSF) using steam-exploded wood (SEW) with a glucose- and cellobiose-fermenting yeast strain, Brettanomyces custersii. An expression to describe the reduction of the relative digestibility during the hydrolysis of the SEW is introduced in the hydrolysis model. The fermentation model also takes two new factors into account, that is, the effects of the inhibitory compounds present in the SEW hydrolysates on the microorganism and the fermenting ability of Brettanomyces custersii, which can use both glucose and cellobiose as carbon sources. The model equations were used to simulate the hydrolysis of the SEW, the fermentation of the SEW hydrolysates, and a batch SSF, and the results were compared with the experimental data. The model was found to be capable of representing ethanol production over a range of substrate concentrations. Accordingly, the limiting factors in ethanol production by SSF under the high concentration of the SEW were identified as the effect of inhibitory compounds present in the SEW, the enzyme deactivation, and a limitation in the digestibility based on the physical condition of the substrate.

Original languageEnglish
Pages (from-to)598-606
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume11
Issue number4
Publication statusPublished - 2001 Oct 8

Fingerprint

Brettanomyces
Cellobiose
Saccharification
Enzyme kinetics
Steam
Yeast
Fermentation
Glucose
Wood
Ethanol
Yeasts
Hydrolysis
Substrates
Microorganisms
Theoretical Models
Carbon
Enzymes
Mathematical models

Keywords

  • Cellobiose-fermenting yeast
  • Ethanol
  • Inhibitory compounds
  • Modeling
  • SSF
  • Steam-exploded wood

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

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title = "Kinetic modeling of simultaneous saccharification and fermentation for ethanol production using steam-exploded wood with glucose- and cellobiose-fermenting yeast, Brettanomyces custersii",
abstract = "A mathematical model is proposed that can depict the kinetics of simultaneous saccharification and fermentation (SSF) using steam-exploded wood (SEW) with a glucose- and cellobiose-fermenting yeast strain, Brettanomyces custersii. An expression to describe the reduction of the relative digestibility during the hydrolysis of the SEW is introduced in the hydrolysis model. The fermentation model also takes two new factors into account, that is, the effects of the inhibitory compounds present in the SEW hydrolysates on the microorganism and the fermenting ability of Brettanomyces custersii, which can use both glucose and cellobiose as carbon sources. The model equations were used to simulate the hydrolysis of the SEW, the fermentation of the SEW hydrolysates, and a batch SSF, and the results were compared with the experimental data. The model was found to be capable of representing ethanol production over a range of substrate concentrations. Accordingly, the limiting factors in ethanol production by SSF under the high concentration of the SEW were identified as the effect of inhibitory compounds present in the SEW, the enzyme deactivation, and a limitation in the digestibility based on the physical condition of the substrate.",
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author = "H. Moon and Kim, {J. S.} and Oh, {K. K.} and Kim, {Seung Wook} and Hong, {S. I.}",
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AU - Kim, J. S.

AU - Oh, K. K.

AU - Kim, Seung Wook

AU - Hong, S. I.

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AB - A mathematical model is proposed that can depict the kinetics of simultaneous saccharification and fermentation (SSF) using steam-exploded wood (SEW) with a glucose- and cellobiose-fermenting yeast strain, Brettanomyces custersii. An expression to describe the reduction of the relative digestibility during the hydrolysis of the SEW is introduced in the hydrolysis model. The fermentation model also takes two new factors into account, that is, the effects of the inhibitory compounds present in the SEW hydrolysates on the microorganism and the fermenting ability of Brettanomyces custersii, which can use both glucose and cellobiose as carbon sources. The model equations were used to simulate the hydrolysis of the SEW, the fermentation of the SEW hydrolysates, and a batch SSF, and the results were compared with the experimental data. The model was found to be capable of representing ethanol production over a range of substrate concentrations. Accordingly, the limiting factors in ethanol production by SSF under the high concentration of the SEW were identified as the effect of inhibitory compounds present in the SEW, the enzyme deactivation, and a limitation in the digestibility based on the physical condition of the substrate.

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