Kinetic study of the lipase-catalyzed interesterification of triolein and stearic acid in nonpolar media

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The kinetics of the interesterification of triolein and stearic acid catalyzed by immobilized Rhizopus delemar lipase were studied in a batch operation. In order to clarify the mechanisms of this reaction, three models are discussed under various conditions in terms of the ratio of triolein and stearic acid. The rate constants involved in the proposed model were determined by combining the numerical Gauss-elemination method, and the trial-and-error method so as to fit the calculated results with the experimental data. The accuracy of the obtained rate constants was confirmed after they were substituted for simultaneous differential equations and the equations simulated using an adaptive step-size Runge-Kutta method. Finally, the model which agrees with the calculated results and the experimental data was selected.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume30
Issue number1
Publication statusPublished - 1997 Jan 31

Fingerprint

Triolein
Lipase
Kinetics
Rate constants
Rhizopus
Runge Kutta methods
Differential equations
stearic acid

Keywords

  • 1,3-specific lipase
  • Kinetics
  • Modeling
  • Rate constants
  • Simulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

@article{f90b240e5d4e43269a005bf1e0ec5063,
title = "Kinetic study of the lipase-catalyzed interesterification of triolein and stearic acid in nonpolar media",
abstract = "The kinetics of the interesterification of triolein and stearic acid catalyzed by immobilized Rhizopus delemar lipase were studied in a batch operation. In order to clarify the mechanisms of this reaction, three models are discussed under various conditions in terms of the ratio of triolein and stearic acid. The rate constants involved in the proposed model were determined by combining the numerical Gauss-elemination method, and the trial-and-error method so as to fit the calculated results with the experimental data. The accuracy of the obtained rate constants was confirmed after they were substituted for simultaneous differential equations and the equations simulated using an adaptive step-size Runge-Kutta method. Finally, the model which agrees with the calculated results and the experimental data was selected.",
keywords = "1,3-specific lipase, Kinetics, Modeling, Rate constants, Simulation",
author = "Chi, {Young Min}",
year = "1997",
month = "1",
day = "31",
language = "English",
volume = "30",
pages = "7--12",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "1",

}

TY - JOUR

T1 - Kinetic study of the lipase-catalyzed interesterification of triolein and stearic acid in nonpolar media

AU - Chi, Young Min

PY - 1997/1/31

Y1 - 1997/1/31

N2 - The kinetics of the interesterification of triolein and stearic acid catalyzed by immobilized Rhizopus delemar lipase were studied in a batch operation. In order to clarify the mechanisms of this reaction, three models are discussed under various conditions in terms of the ratio of triolein and stearic acid. The rate constants involved in the proposed model were determined by combining the numerical Gauss-elemination method, and the trial-and-error method so as to fit the calculated results with the experimental data. The accuracy of the obtained rate constants was confirmed after they were substituted for simultaneous differential equations and the equations simulated using an adaptive step-size Runge-Kutta method. Finally, the model which agrees with the calculated results and the experimental data was selected.

AB - The kinetics of the interesterification of triolein and stearic acid catalyzed by immobilized Rhizopus delemar lipase were studied in a batch operation. In order to clarify the mechanisms of this reaction, three models are discussed under various conditions in terms of the ratio of triolein and stearic acid. The rate constants involved in the proposed model were determined by combining the numerical Gauss-elemination method, and the trial-and-error method so as to fit the calculated results with the experimental data. The accuracy of the obtained rate constants was confirmed after they were substituted for simultaneous differential equations and the equations simulated using an adaptive step-size Runge-Kutta method. Finally, the model which agrees with the calculated results and the experimental data was selected.

KW - 1,3-specific lipase

KW - Kinetics

KW - Modeling

KW - Rate constants

KW - Simulation

UR - http://www.scopus.com/inward/record.url?scp=3042983709&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042983709&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:3042983709

VL - 30

SP - 7

EP - 12

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 1

ER -