Kinetics of serological response in patients with severe fever with thrombocytopenia syndrome

Sang Hyun Ra, Min Jae Kim, Min Chul Kim, Se Yoon Park, Seong Yeon Park, Yong Pil Chong, Sang Oh Lee, Sang Ho Choi, Yang Soo Kim, Keun Hwa Lee, Sung Han Kim, Sun Ho Kee

Research output: Contribution to journalArticlepeer-review

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in their plasma using reverse transcription polymerase chain reaction. SFTSV-specific IgG and IgM were measured using immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). We found that SFTSV-specific IgG was detected at days 5–9 after symptom onset, and its titer was rising during the course of disease. SFTSV-specific IgM titer peaked at around week 2–3 from symptom onset. The SFTSV-specific seropositive rates for days 5–9, 10–14, 15–19, and 20–24 from symptom onset using IFA and ELISA were 63%, 76%, 90%, and 100%, and 58%, 86%, 100%, and 100%, respectively, for IgG, whereas they were 32%, 62%, 80%, and 100%, and 53%, 62%, 70%, and 100%, respectively, for IgM. The delayed IgM response could be attributed to the low sensitivity of SFTSV-specific IgM IFA or ELISA and/or impaired immune responses. The IgM test using IFA or ELISA that we used in this study is, therefore, insufficient for the early diagnosis of SFTS.

Original languageEnglish
Article number6
JournalViruses
Volume13
Issue number1
DOIs
Publication statusPublished - 2021 Jan

Keywords

  • Enzyme-linked immunosorbent assay (ELISA)
  • Immunofluorescence assay (IFA)
  • Immunoglobulin G (IgG)
  • Immunoglobulin M (IgM)
  • Severe fever with thrombocytopenia syndrome (SFTS)
  • Viral load

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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