L-Fucose Synthesis Using a Halo- and Thermophilic L-Fucose Isomerase from Polyextremophilic Halothermothrix orenii

In Jung Kim, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

L-Fucose isomerase (L-FucI)-mediated isomerization is a promising biotechnological approach for synthesizing various rare sugars of industrial significance, including L-fucose. Extremozymes that can retain their functional conformation under extreme conditions, such as high temperature and salinity, offer favorable applications in bioprocesses that accompany harsh conditions. To date, only one thermophilic L-FucI has been characterized for L-fucose synthesis. Here, we report L-FucI from Halothermothrix orenii (HoFucI) which exhibits both halophilic and thermophilic properties. When evaluated under various biochemical conditions, HoFucI exhibited optimal activities at 50–60 °C and pH 7 with 0.5–1 M NaCl in the presence of 1 mM Mn2+ as a cofactor. The results obtained here show a unique feature of HoFucI as a polyextremozyme, which facilitates the biotechnological production of L-fucose using this enzyme.

Original languageEnglish
Article number4029
JournalApplied Sciences (Switzerland)
Volume12
Issue number8
DOIs
Publication statusPublished - 2022 Apr 1

Keywords

  • Halothermothrix orenii
  • L-fucose
  • L-fucose isomerase
  • L-fuculose
  • extremophile
  • halothermophilic bacteria

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

Fingerprint

Dive into the research topics of 'L-Fucose Synthesis Using a Halo- and Thermophilic L-Fucose Isomerase from Polyextremophilic Halothermothrix orenii'. Together they form a unique fingerprint.

Cite this