Label-free and high-sensitive detection of Kirsten rat sarcoma viral oncogene homolog and epidermal growth factor receptor mutation using Kelvin probe force microscopy

Kuewhan Jang, Jaeyeong Choia, Chanho Park, Sung Soo Na

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Assessment of Kirsten rat sarcoma viral oncogene homolog (KRAS) and epidermal growth factor receptor (EGFR) mutations are essential for targeted therapies of patients with non–small–cell lung cancer. In this report, we propose a label-free and high-sensitive detection method of KRAS and EGFR mutations using KPFM and a gold nanoparticle (AuNP)–based platform that densely adsorbs probe DNA and minimizes the sensing area. The detection is based on the evaluation of the surface potential of each AuNP. When AuNPs are modified with probe DNA (AuNP–pDNA), the surface potential is shifted towards the negative potential due to the negatively charged DNA backbone. When AuNP–pDNA further captures target mutant DNA through DNA hybridization, an additional surface potential shift occurs. The platform is able to detect KRAS mutant DNA (13 mer) and EGFR mutant DNA (84 mer) with a limit of detection (LOD) of 3.3 pM. Furthermore, the platform is able to detect selectively the KRAS mutant DNA from its wild-type DNA. Our proposed label-free and high-sensitive KPFM method has shown potential glimpses of a personalized medical diagnosis for cancer patients.

Original languageEnglish
Pages (from-to)222-228
Number of pages7
JournalBiosensors and Bioelectronics
Volume87
DOIs
Publication statusPublished - 2017 Jan 15

Fingerprint

Atomic Force Microscopy
Oncogenes
Epidermal Growth Factor Receptor
Sarcoma
Rats
Labels
Microscopic examination
DNA
Mutation
DNA Probes
Surface potential
Epidermal Growth Factor
Gold
Nanoparticles
Limit of Detection
Lung Neoplasms
Neoplasms

Keywords

  • Detection
  • DNA
  • Epidermal growth factor receptor (EGFR)
  • Gold nanoparticle (AuNP)
  • Kelvin probe force microscopy (KPFM)
  • Kirsten rat sarcoma viral oncogene homolog (KRAS)

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

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abstract = "Assessment of Kirsten rat sarcoma viral oncogene homolog (KRAS) and epidermal growth factor receptor (EGFR) mutations are essential for targeted therapies of patients with non–small–cell lung cancer. In this report, we propose a label-free and high-sensitive detection method of KRAS and EGFR mutations using KPFM and a gold nanoparticle (AuNP)–based platform that densely adsorbs probe DNA and minimizes the sensing area. The detection is based on the evaluation of the surface potential of each AuNP. When AuNPs are modified with probe DNA (AuNP–pDNA), the surface potential is shifted towards the negative potential due to the negatively charged DNA backbone. When AuNP–pDNA further captures target mutant DNA through DNA hybridization, an additional surface potential shift occurs. The platform is able to detect KRAS mutant DNA (13 mer) and EGFR mutant DNA (84 mer) with a limit of detection (LOD) of 3.3 pM. Furthermore, the platform is able to detect selectively the KRAS mutant DNA from its wild-type DNA. Our proposed label-free and high-sensitive KPFM method has shown potential glimpses of a personalized medical diagnosis for cancer patients.",
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