Label-free detection of bacterial RNA using polydiacetylene-based biochip

Moo Kyung Park, Kyung Woo Kim, Dong June Ahn, Min Kyu Oh

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We developed a simple and effective polydiacetylene-based, label-free multiplex DNA chip for the detection of various pathogenic microorganisms. A novel immobilization method of PDA vesicles on glass slides was exploited using α-cyclodextrin (α-CD). The surface topography of the efficiently immobilized PDA vesicles was confirmed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Then, oligonucleotides complementary to rRNAs of three pathogenic bacteria were conjugated to the PDA vesicles. Finally, crude lysate of pathogenic bacteria was applied to the PDA biochip. The pathogenic bacteria were specifically detected by DNA-RNA hybridization in an hour. The new PDA sensor was effective in detecting multiple pathogenic bacteria easily and accurately without rigorous purification, amplification, and labeling of their genetic components.

Original languageEnglish
Pages (from-to)44-49
Number of pages6
JournalBiosensors and Bioelectronics
Volume35
Issue number1
DOIs
Publication statusPublished - 2012 May 15

Keywords

  • Immobilization
  • Label-free
  • Multiplex detection
  • Pathogenic bacteria
  • Polydiacetylene

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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