Label-free imaging of membrane potential using membrane electromotility

Seungeun Oh, Christopher Fang-Yen, Wonshik Choi, Zahid Yaqoob, Dan Fu, Yongkeun Park, Ramachandra R. Dassari, Michael S. Feld

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Electrical activity may cause observable changes in a cell's structure in the absence of exogenous reporter molecules. In this work, we report a low-coherence interferometric microscopy technique that can detect an optical signal correlated with the membrane potential changes in individual mammalian cells without exogenous labels. By measuring milliradian-scale phase shifts in the transmitted light, we can detect changes in the cells' membrane potential. We find that the observed optical signals are due to membrane electromotility, which causes the cells to deform in response to the membrane potential changes. We demonstrate wide-field imaging of the propagation of electrical stimuli in gap-junction-coupled cell networks. Membrane electromotility-induced cell deformation may be useful as a reporter of electrical activity.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalBiophysical Journal
Volume103
Issue number1
DOIs
Publication statusPublished - 2012 Jul 3

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Membrane Potentials
Membranes
Gap Junctions
Microscopy
Cell Membrane
Light

ASJC Scopus subject areas

  • Biophysics

Cite this

Oh, S., Fang-Yen, C., Choi, W., Yaqoob, Z., Fu, D., Park, Y., ... Feld, M. S. (2012). Label-free imaging of membrane potential using membrane electromotility. Biophysical Journal, 103(1), 11-18. https://doi.org/10.1016/j.bpj.2012.05.020

Label-free imaging of membrane potential using membrane electromotility. / Oh, Seungeun; Fang-Yen, Christopher; Choi, Wonshik; Yaqoob, Zahid; Fu, Dan; Park, Yongkeun; Dassari, Ramachandra R.; Feld, Michael S.

In: Biophysical Journal, Vol. 103, No. 1, 03.07.2012, p. 11-18.

Research output: Contribution to journalArticle

Oh, S, Fang-Yen, C, Choi, W, Yaqoob, Z, Fu, D, Park, Y, Dassari, RR & Feld, MS 2012, 'Label-free imaging of membrane potential using membrane electromotility', Biophysical Journal, vol. 103, no. 1, pp. 11-18. https://doi.org/10.1016/j.bpj.2012.05.020
Oh, Seungeun ; Fang-Yen, Christopher ; Choi, Wonshik ; Yaqoob, Zahid ; Fu, Dan ; Park, Yongkeun ; Dassari, Ramachandra R. ; Feld, Michael S. / Label-free imaging of membrane potential using membrane electromotility. In: Biophysical Journal. 2012 ; Vol. 103, No. 1. pp. 11-18.
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