Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

Keon Woo Kwon, Sung Sik Choi, Sang Ho Lee, Byungkyu Kim, Se Na Lee, Min Cheol Park, Pilnam Kim, Se Yon Hwang, Kahp Y. Suh

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

A label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the adhesion difference between normal epithelial and cancer cells, flat or nanostructured polymer surfaces (400 nm pillars, 400 nm perpendicular, or 400 nm parallel lines) were constructed on the bottom of polydimethylsiloxane (PDMS) microfluidic channels in a parallel fashion using a UV-assisted capillary moulding technique. The adhesion of human breast epithelial cells (MCF10A) and cancer cells (MCF7) on each channel was independently measured based on detachment assays where the adherent cells were counted with increasing flow rate after a pre-culture for a period of time (e.g., one, two, and four hours). It was found that MCF10A cells showed higher adhesion than MCF7 cells regardless of culture time and surface nanotopography at all flow rates, resulting in label-free separation and enrichment of cancer cells. For the cell types used in our study, an optimum separation was found for 2 hours pre-culture on the 400 nm perpendicular line pattern followed by flow-induced detachment at a flow rate of 200 μl min-1. The fraction of MCF7 cells was increased from 0.36 ± 0.04 to 0.83 ± 0.04 under these optimized conditions.

Original languageEnglish
Pages (from-to)1461-1468
Number of pages8
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume7
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

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Microfluidics
Cell Adhesion
Labels
Adhesion
Cells
Breast Neoplasms
MCF-7 Cells
Flow rate
Epithelial Cells
Cell adhesion
Polydimethylsiloxane
Neoplasms
Molding
Assays
Polymers
Breast
Cell Culture Techniques

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference. / Kwon, Keon Woo; Choi, Sung Sik; Lee, Sang Ho; Kim, Byungkyu; Lee, Se Na; Park, Min Cheol; Kim, Pilnam; Hwang, Se Yon; Suh, Kahp Y.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 7, No. 11, 01.11.2007, p. 1461-1468.

Research output: Contribution to journalArticle

Kwon, Keon Woo ; Choi, Sung Sik ; Lee, Sang Ho ; Kim, Byungkyu ; Lee, Se Na ; Park, Min Cheol ; Kim, Pilnam ; Hwang, Se Yon ; Suh, Kahp Y. / Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2007 ; Vol. 7, No. 11. pp. 1461-1468.
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