Lamb wave-based molecular diagnosis using DNA hydrogel formation by rolling circle amplification (RCA) process

Jeonghun Nam, Woong Sik Jang, Jisu Kim, Hyukjin Lee, Chae Seung Lim

Research output: Contribution to journalArticle

Abstract

Recent developments in microfluidics enable the lab-on-a-chip-based molecular diagnosis. Rapid and accurate diagnosis of infectious diseases is critical for preventing the transmission of the disease. Here, we characterize a Lamb wave-based device using various parameters including the contact angle and viscosity of the sample droplet, the applied voltage, and the temperature increase. Additionally, we demonstrate the functionality of the Lamb wave-based device in clinical application. Optimal temperature for rolling circle amplification (RCA) process is 30 °C, and it was achieved by Lamb wave generation at 17 V. Gene amplification due to RCA process could be detected by viscosity increase due to DNA hydrogel formation in a sample droplet, which induced the acoustic streaming velocity of suspended particles to be decreased. In our Lamb wave-based device, isothermal amplification of target nucleic acids could be successfully detected within 30 min using 10 μL of sessile droplet, and was validated by comparing that of commercial real-time fluorescence analysis. Our device enables simple and low-cost molecular diagnosis, which can be applied to resource-limited clinical settings.

Original languageEnglish
Article number111496
JournalBiosensors and Bioelectronics
Volume142
DOIs
Publication statusPublished - 2019 Oct 1

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Hydrogel
Hydrogels
Surface waves
Amplification
DNA
Equipment and Supplies
Viscosity
Acoustic streaming
Lab-on-a-chip
Temperature
Microfluidics
Gene Amplification
Nucleic acids
Acoustics
Nucleic Acids
Contact angle
Communicable Diseases
Fluorescence
Costs and Cost Analysis
Electric potential

Keywords

  • Acoustic streaming
  • DNA hydrogel
  • Gene amplification
  • Lamb wave
  • Molecular diagnosis

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Lamb wave-based molecular diagnosis using DNA hydrogel formation by rolling circle amplification (RCA) process. / Nam, Jeonghun; Jang, Woong Sik; Kim, Jisu; Lee, Hyukjin; Lim, Chae Seung.

In: Biosensors and Bioelectronics, Vol. 142, 111496, 01.10.2019.

Research output: Contribution to journalArticle

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