Large and Externally Positioned Ligand-Coated Nanopatches Facilitate the Adhesion-Dependent Regenerative Polarization of Host Macrophages

Sunhong Min, Yoo Sang Jeon, Hyojun Choi, Chandra Khatua, Na Li, Gunhyu Bae, Hee Joon Jung, Yuri Kim, Hyunsik Hong, Jeongeun Shin, Min Jun Ko, Han Seok Ko, Taesoon Kim, Jun Hwan Moon, Jae Jun Song, Vinayak P. Dravid, Young Keun Kim, Heemin Kang

Research output: Contribution to journalArticlepeer-review

Abstract

Macrophages can associate with extracellular matrix (ECM) demonstrating nanosequenced cell-adhesive RGD ligand. In this study, we devised barcoded materials composed of RGD-coated gold and RGD-absent iron nanopatches to show various frequencies and position of RGD-coated nanopatches with similar areas of iron and RGD-gold nanopatches that maintain macroscale and nanoscale RGD density invariant. Iron patches were used for substrate coupling. Both large (low frequency) and externally positioned RGD-coated nanopatches stimulated robust attachment in macrophages, compared with small (high frequency) and internally positioned RGD-coated nanopatches, respectively, which mediate their regenerative/anti-inflammatory M2 polarization. The nanobarcodes exhibited stability in vivo. We shed light into designing ligand-engineered nanostructures in an external position to facilitate host cell attachment, thereby eliciting regenerative host responses.

Original languageEnglish
Pages (from-to)7272-7280
Number of pages9
JournalNano Letters
Volume20
Issue number10
DOIs
Publication statusPublished - 2020 Oct 14

Keywords

  • RGD frequency
  • RGD nanopatches
  • macrophage adhesion
  • macrophage polarization

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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