Laticifer tissue-specific activation of the Hevea SRPP promoter in Taraxacum brevicorniculatum and its regulation by light, tapping and cold stress

Sandeep Kumar Tata, Jun Young Choi, Ji Yul Jung, Ka Yung Lim, Jeong Sheop Shin, Stephen Beungtae Ryu

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Hevea brasiliensis is an important plant species currently cultivated for the commercial production of natural rubber. As the demand for rubber continues to increase, it is important to identify alternative sources of natural rubber and to increase plant rubber content using molecular approaches. Taraxacum kok-saghyz, a Russian dandelion, produces natural rubber that is of high quality. In this study, the SMALL RUBBER PARTICLE PROTEIN (SRPP) promoter from H. brasiliensis was characterized to determine its suitability for the expression of latex-specific genes in Taraxacum brevicorniculatum which is another Russian dandelion species of T. kok-saghyz from the similar geographical areas. Studies using transgenic Taraxacum plants carrying the SRPP promoter::β-glucuronidase (GUS) sequence indicate that the SRPP promoter does induce gene expression primarily in laticiferous tissues. Additionally, the promoter was regulated by various external conditions including light, tapping, and cold. These findings suggest that the SRPP promoter will be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of Taraxacum plant species.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalIndustrial Crops and Products
Volume40
Issue number1
DOIs
Publication statusPublished - 2012 Nov

Keywords

  • Hevea brasiliensis
  • Latex
  • Natural rubber
  • SRPP promoter
  • Taraxacum brevicorniculatum
  • Taraxacum kok-saghyz

ASJC Scopus subject areas

  • Agronomy and Crop Science

Fingerprint Dive into the research topics of 'Laticifer tissue-specific activation of the Hevea SRPP promoter in Taraxacum brevicorniculatum and its regulation by light, tapping and cold stress'. Together they form a unique fingerprint.

Cite this