Laticifer tissue-specific activation of the Hevea SRPP promoter in Taraxacum brevicorniculatum and its regulation by light, tapping and cold stress

Sandeep Kumar Tata, Jun Young Choi, Ji Yul Jung, Ka Yung Lim, Jeong Sheop Shin, Stephen Beungtae Ryu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Hevea brasiliensis is an important plant species currently cultivated for the commercial production of natural rubber. As the demand for rubber continues to increase, it is important to identify alternative sources of natural rubber and to increase plant rubber content using molecular approaches. Taraxacum kok-saghyz, a Russian dandelion, produces natural rubber that is of high quality. In this study, the SMALL RUBBER PARTICLE PROTEIN (SRPP) promoter from H. brasiliensis was characterized to determine its suitability for the expression of latex-specific genes in Taraxacum brevicorniculatum which is another Russian dandelion species of T. kok-saghyz from the similar geographical areas. Studies using transgenic Taraxacum plants carrying the SRPP promoter::β-glucuronidase (GUS) sequence indicate that the SRPP promoter does induce gene expression primarily in laticiferous tissues. Additionally, the promoter was regulated by various external conditions including light, tapping, and cold. These findings suggest that the SRPP promoter will be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of Taraxacum plant species.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalIndustrial Crops and Products
Volume40
Issue number1
DOIs
Publication statusPublished - 2012 Nov 1

Fingerprint

Hevea
Taraxacum
cold stress
rubber
promoter regions
Taraxacum kok-saghyz
proteins
Hevea brasiliensis
tissues
gene expression
latex
genetically modified organisms

Keywords

  • Hevea brasiliensis
  • Latex
  • Natural rubber
  • SRPP promoter
  • Taraxacum brevicorniculatum
  • Taraxacum kok-saghyz

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Laticifer tissue-specific activation of the Hevea SRPP promoter in Taraxacum brevicorniculatum and its regulation by light, tapping and cold stress. / Tata, Sandeep Kumar; Choi, Jun Young; Jung, Ji Yul; Lim, Ka Yung; Shin, Jeong Sheop; Ryu, Stephen Beungtae.

In: Industrial Crops and Products, Vol. 40, No. 1, 01.11.2012, p. 219-224.

Research output: Contribution to journalArticle

Tata, Sandeep Kumar ; Choi, Jun Young ; Jung, Ji Yul ; Lim, Ka Yung ; Shin, Jeong Sheop ; Ryu, Stephen Beungtae. / Laticifer tissue-specific activation of the Hevea SRPP promoter in Taraxacum brevicorniculatum and its regulation by light, tapping and cold stress. In: Industrial Crops and Products. 2012 ; Vol. 40, No. 1. pp. 219-224.
@article{7037abd9b3f44f80a2201723a3aed6d7,
title = "Laticifer tissue-specific activation of the Hevea SRPP promoter in Taraxacum brevicorniculatum and its regulation by light, tapping and cold stress",
abstract = "Hevea brasiliensis is an important plant species currently cultivated for the commercial production of natural rubber. As the demand for rubber continues to increase, it is important to identify alternative sources of natural rubber and to increase plant rubber content using molecular approaches. Taraxacum kok-saghyz, a Russian dandelion, produces natural rubber that is of high quality. In this study, the SMALL RUBBER PARTICLE PROTEIN (SRPP) promoter from H. brasiliensis was characterized to determine its suitability for the expression of latex-specific genes in Taraxacum brevicorniculatum which is another Russian dandelion species of T. kok-saghyz from the similar geographical areas. Studies using transgenic Taraxacum plants carrying the SRPP promoter::β-glucuronidase (GUS) sequence indicate that the SRPP promoter does induce gene expression primarily in laticiferous tissues. Additionally, the promoter was regulated by various external conditions including light, tapping, and cold. These findings suggest that the SRPP promoter will be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of Taraxacum plant species.",
keywords = "Hevea brasiliensis, Latex, Natural rubber, SRPP promoter, Taraxacum brevicorniculatum, Taraxacum kok-saghyz",
author = "Tata, {Sandeep Kumar} and Choi, {Jun Young} and Jung, {Ji Yul} and Lim, {Ka Yung} and Shin, {Jeong Sheop} and Ryu, {Stephen Beungtae}",
year = "2012",
month = "11",
day = "1",
doi = "10.1016/j.indcrop.2012.03.012",
language = "English",
volume = "40",
pages = "219--224",
journal = "Industrial Crops and Products",
issn = "0926-6690",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Laticifer tissue-specific activation of the Hevea SRPP promoter in Taraxacum brevicorniculatum and its regulation by light, tapping and cold stress

AU - Tata, Sandeep Kumar

AU - Choi, Jun Young

AU - Jung, Ji Yul

AU - Lim, Ka Yung

AU - Shin, Jeong Sheop

AU - Ryu, Stephen Beungtae

PY - 2012/11/1

Y1 - 2012/11/1

N2 - Hevea brasiliensis is an important plant species currently cultivated for the commercial production of natural rubber. As the demand for rubber continues to increase, it is important to identify alternative sources of natural rubber and to increase plant rubber content using molecular approaches. Taraxacum kok-saghyz, a Russian dandelion, produces natural rubber that is of high quality. In this study, the SMALL RUBBER PARTICLE PROTEIN (SRPP) promoter from H. brasiliensis was characterized to determine its suitability for the expression of latex-specific genes in Taraxacum brevicorniculatum which is another Russian dandelion species of T. kok-saghyz from the similar geographical areas. Studies using transgenic Taraxacum plants carrying the SRPP promoter::β-glucuronidase (GUS) sequence indicate that the SRPP promoter does induce gene expression primarily in laticiferous tissues. Additionally, the promoter was regulated by various external conditions including light, tapping, and cold. These findings suggest that the SRPP promoter will be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of Taraxacum plant species.

AB - Hevea brasiliensis is an important plant species currently cultivated for the commercial production of natural rubber. As the demand for rubber continues to increase, it is important to identify alternative sources of natural rubber and to increase plant rubber content using molecular approaches. Taraxacum kok-saghyz, a Russian dandelion, produces natural rubber that is of high quality. In this study, the SMALL RUBBER PARTICLE PROTEIN (SRPP) promoter from H. brasiliensis was characterized to determine its suitability for the expression of latex-specific genes in Taraxacum brevicorniculatum which is another Russian dandelion species of T. kok-saghyz from the similar geographical areas. Studies using transgenic Taraxacum plants carrying the SRPP promoter::β-glucuronidase (GUS) sequence indicate that the SRPP promoter does induce gene expression primarily in laticiferous tissues. Additionally, the promoter was regulated by various external conditions including light, tapping, and cold. These findings suggest that the SRPP promoter will be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of Taraxacum plant species.

KW - Hevea brasiliensis

KW - Latex

KW - Natural rubber

KW - SRPP promoter

KW - Taraxacum brevicorniculatum

KW - Taraxacum kok-saghyz

UR - http://www.scopus.com/inward/record.url?scp=84859405328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859405328&partnerID=8YFLogxK

U2 - 10.1016/j.indcrop.2012.03.012

DO - 10.1016/j.indcrop.2012.03.012

M3 - Article

VL - 40

SP - 219

EP - 224

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

IS - 1

ER -