Abstract
Hevea brasiliensis is an important plant species currently cultivated for the commercial production of natural rubber. As the demand for rubber continues to increase, it is important to identify alternative sources of natural rubber and to increase plant rubber content using molecular approaches. Taraxacum kok-saghyz, a Russian dandelion, produces natural rubber that is of high quality. In this study, the SMALL RUBBER PARTICLE PROTEIN (SRPP) promoter from H. brasiliensis was characterized to determine its suitability for the expression of latex-specific genes in Taraxacum brevicorniculatum which is another Russian dandelion species of T. kok-saghyz from the similar geographical areas. Studies using transgenic Taraxacum plants carrying the SRPP promoter::β-glucuronidase (GUS) sequence indicate that the SRPP promoter does induce gene expression primarily in laticiferous tissues. Additionally, the promoter was regulated by various external conditions including light, tapping, and cold. These findings suggest that the SRPP promoter will be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of Taraxacum plant species.
| Original language | English |
|---|---|
| Pages (from-to) | 219-224 |
| Number of pages | 6 |
| Journal | Industrial Crops and Products |
| Volume | 40 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2012 Nov 1 |
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Keywords
- Hevea brasiliensis
- Latex
- Natural rubber
- SRPP promoter
- Taraxacum brevicorniculatum
- Taraxacum kok-saghyz
ASJC Scopus subject areas
- Agronomy and Crop Science
Cite this
Laticifer tissue-specific activation of the Hevea SRPP promoter in Taraxacum brevicorniculatum and its regulation by light, tapping and cold stress. / Tata, Sandeep Kumar; Choi, Jun Young; Jung, Ji Yul; Lim, Ka Yung; Shin, Jeong Sheop; Ryu, Stephen Beungtae.
In: Industrial Crops and Products, Vol. 40, No. 1, 01.11.2012, p. 219-224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Laticifer tissue-specific activation of the Hevea SRPP promoter in Taraxacum brevicorniculatum and its regulation by light, tapping and cold stress
AU - Tata, Sandeep Kumar
AU - Choi, Jun Young
AU - Jung, Ji Yul
AU - Lim, Ka Yung
AU - Shin, Jeong Sheop
AU - Ryu, Stephen Beungtae
PY - 2012/11/1
Y1 - 2012/11/1
N2 - Hevea brasiliensis is an important plant species currently cultivated for the commercial production of natural rubber. As the demand for rubber continues to increase, it is important to identify alternative sources of natural rubber and to increase plant rubber content using molecular approaches. Taraxacum kok-saghyz, a Russian dandelion, produces natural rubber that is of high quality. In this study, the SMALL RUBBER PARTICLE PROTEIN (SRPP) promoter from H. brasiliensis was characterized to determine its suitability for the expression of latex-specific genes in Taraxacum brevicorniculatum which is another Russian dandelion species of T. kok-saghyz from the similar geographical areas. Studies using transgenic Taraxacum plants carrying the SRPP promoter::β-glucuronidase (GUS) sequence indicate that the SRPP promoter does induce gene expression primarily in laticiferous tissues. Additionally, the promoter was regulated by various external conditions including light, tapping, and cold. These findings suggest that the SRPP promoter will be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of Taraxacum plant species.
AB - Hevea brasiliensis is an important plant species currently cultivated for the commercial production of natural rubber. As the demand for rubber continues to increase, it is important to identify alternative sources of natural rubber and to increase plant rubber content using molecular approaches. Taraxacum kok-saghyz, a Russian dandelion, produces natural rubber that is of high quality. In this study, the SMALL RUBBER PARTICLE PROTEIN (SRPP) promoter from H. brasiliensis was characterized to determine its suitability for the expression of latex-specific genes in Taraxacum brevicorniculatum which is another Russian dandelion species of T. kok-saghyz from the similar geographical areas. Studies using transgenic Taraxacum plants carrying the SRPP promoter::β-glucuronidase (GUS) sequence indicate that the SRPP promoter does induce gene expression primarily in laticiferous tissues. Additionally, the promoter was regulated by various external conditions including light, tapping, and cold. These findings suggest that the SRPP promoter will be a useful molecular tool for the manipulation of gene expression in the laticiferous tissues of Taraxacum plant species.
KW - Hevea brasiliensis
KW - Latex
KW - Natural rubber
KW - SRPP promoter
KW - Taraxacum brevicorniculatum
KW - Taraxacum kok-saghyz
UR - http://www.scopus.com/inward/record.url?scp=84859405328&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859405328&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2012.03.012
DO - 10.1016/j.indcrop.2012.03.012
M3 - Article
VL - 40
SP - 219
EP - 224
JO - Industrial Crops and Products
JF - Industrial Crops and Products
SN - 0926-6690
IS - 1
ER -