Laxative effect of probiotic chocolate on loperamide-induced constipation in rats

Ju Young Eor, Pei Lei Tan, Sei Mi Lim, Da Hye Choi, Seok Min Yoon, Si Young Yang, Sae Hun Kim

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


The fecal morphology, defecation frequency, bowel function, intestinal motility, and fecal bacterial composition were evaluated to investigate the laxative effect of probiotic chocolate containing Streptococcus thermophilus MG510 and Lactobacillus plantarum LRCC5193 (LYC) on loperamide-induced constipated rats. Daily oral administration of LYC in constipated rats for two weeks was shown to significantly increase (n = 14) the defecation frequency, fecal moisture content, and relative abundance of fecal Lactobacillus and Faecalibacterium prausnitzii. Moreover, histological analysis of the distal colon of constipated rats revealed that LYC treatment can also increase the thickness of the colonic mucosa and muscle layers, and crypt of Lieberkühn. LYC also significantly increased (n = 5) the intestinal motility and modulated (n = 9) mRNA expression levels of colonic ZO-1 and Cldn-1 in the constipated rats. Altogether, these results demonstrate that probiotic chocolate has potential as a dietary adjunct for the treatment of constipation.

Original languageEnglish
Pages (from-to)1173-1182
Number of pages10
JournalFood Research International
Publication statusPublished - 2019 Feb


  • Constipation
  • Gastrointestinal health
  • Lactobacillus plantarum LRCC5193
  • Probiotic chocolate
  • Streptococcus thermophilus MG510

ASJC Scopus subject areas

  • Food Science


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