Legionella lipoprotein activates toll-like receptor 2 and induces cytokine production and expression of costimulatory molecules in peritoneal macrophages

Ki Shim Ho, Yeon Kim Jeoung, Jeong Kim Mi, Sun Sim Hee, Dae Won Park, Jang Wook Sohn, Ja Kim Min

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Legionella bacterium, an intracellular pathogen of mononuclear phagocytes, causes acute fatal pneumonia, especially in patients with impaired cellular immune responses. Until recently, however, the toll-like receptor (TLR) engagement of bacterial proteins derived from Legionella is uncertain. We previously showed that a 19-kDa highly conserved peptidoglycan-associated lipoprotein (PAL) of Legionella pneumophila induced the PAL-specific B cell and T cell responses in mice. In this study, we observed that the rPAL antigen of L. pneumophila, as an effector molecule, activated murine macrophages via TLR2 and produced proinflammatory cytokines such as IL-6 and TNF-α. In both BALB/c and TLR4-deficient C3H/HeJ mice, pretreatment of macrophages with anti-TLR2 mAb showed severely impaired cytokine production in response to the rPAL. In addition, in vitro the rPAL treatment increased the cell surface expression of CD40, CD80, CD86 and MHC I/II molecules. We further showed that the synthetic CpG-oligodeoxynucleotides (CpG ODN) coadministered with the rPAL enhanced IL-12 and IL-6 production and expression of CD40, CD80 and MHC II compared to the rPAL treatment alone. In conclusions, these results indicate that Legionella PAL might activate macrophages via a TLR2-dependent mechanism which thus induce cytokine production and expression of costimulatory and MHC molecules.

Original languageEnglish
Pages (from-to)687-694
Number of pages8
JournalExperimental and Molecular Medicine
Volume41
Issue number10
DOIs
Publication statusPublished - 2009 Oct 31

Fingerprint

Toll-Like Receptor 2
Legionella
Peptidoglycan
Macrophages
Peritoneal Macrophages
Lipoproteins
Cytokines
Molecules
Interleukin-6
Legionella pneumophila
Bacterial Proteins
T-cells
Inbred C3H Mouse
Oligodeoxyribonucleotides
Toll-Like Receptors
Pathogens
Interleukin-12
Phagocytes
Cellular Immunity
Pneumonia

Keywords

  • Legionella pneumophila
  • Legionnaires' disease
  • Lipoproteins
  • Macrophages, peritoneal
  • Toll-like receptors

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Clinical Biochemistry

Cite this

Legionella lipoprotein activates toll-like receptor 2 and induces cytokine production and expression of costimulatory molecules in peritoneal macrophages. / Ho, Ki Shim; Jeoung, Yeon Kim; Mi, Jeong Kim; Hee, Sun Sim; Park, Dae Won; Sohn, Jang Wook; Min, Ja Kim.

In: Experimental and Molecular Medicine, Vol. 41, No. 10, 31.10.2009, p. 687-694.

Research output: Contribution to journalArticle

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