Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid

Hyun-Seuk Moon, Hong Gu Lee, Ji Hye Seo, Chung Soo Chung, Ding Ding Guo, Tae Gyu Kim, Yun Jaie Choi, Chong Su Cho

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.

Original languageEnglish
Pages (from-to)955-960
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number4
DOIs
Publication statusPublished - 2007 May 18
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 2
Leptin
Adipocytes
3T3-L1 Cells
Adipogenesis
Linoleic Acid
Matrix Metalloproteinases
Metabolism
Adipose Tissue
Triglycerides
Proteins
Obesity
Body Weight
trans-10,cis-12-conjugated linoleic acid
Hormones
Tissue

Keywords

  • Conjugated linoleic acid
  • Leptin
  • Lipid accumulation
  • Matrix metalloproteinase-2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid. / Moon, Hyun-Seuk; Lee, Hong Gu; Seo, Ji Hye; Chung, Chung Soo; Guo, Ding Ding; Kim, Tae Gyu; Choi, Yun Jaie; Cho, Chong Su.

In: Biochemical and Biophysical Research Communications, Vol. 356, No. 4, 18.05.2007, p. 955-960.

Research output: Contribution to journalArticle

Moon, Hyun-Seuk ; Lee, Hong Gu ; Seo, Ji Hye ; Chung, Chung Soo ; Guo, Ding Ding ; Kim, Tae Gyu ; Choi, Yun Jaie ; Cho, Chong Su. / Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 356, No. 4. pp. 955-960.
@article{2799e8d2d47c463e8f8ec764e73f41ff,
title = "Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid",
abstract = "It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.",
keywords = "Conjugated linoleic acid, Leptin, Lipid accumulation, Matrix metalloproteinase-2",
author = "Hyun-Seuk Moon and Lee, {Hong Gu} and Seo, {Ji Hye} and Chung, {Chung Soo} and Guo, {Ding Ding} and Kim, {Tae Gyu} and Choi, {Yun Jaie} and Cho, {Chong Su}",
year = "2007",
month = "5",
day = "18",
doi = "10.1016/j.bbrc.2007.03.068",
language = "English",
volume = "356",
pages = "955--960",
journal = "The BMJ",
issn = "0730-6512",
publisher = "Kluwer Academic Publishers",
number = "4",

}

TY - JOUR

T1 - Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid

AU - Moon, Hyun-Seuk

AU - Lee, Hong Gu

AU - Seo, Ji Hye

AU - Chung, Chung Soo

AU - Guo, Ding Ding

AU - Kim, Tae Gyu

AU - Choi, Yun Jaie

AU - Cho, Chong Su

PY - 2007/5/18

Y1 - 2007/5/18

N2 - It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.

AB - It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.

KW - Conjugated linoleic acid

KW - Leptin

KW - Lipid accumulation

KW - Matrix metalloproteinase-2

UR - http://www.scopus.com/inward/record.url?scp=34047097866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34047097866&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.03.068

DO - 10.1016/j.bbrc.2007.03.068

M3 - Article

VL - 356

SP - 955

EP - 960

JO - The BMJ

JF - The BMJ

SN - 0730-6512

IS - 4

ER -