Abstract
It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.
| Original language | English |
|---|---|
| Pages (from-to) | 955-960 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 356 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2007 May 18 |
| Externally published | Yes |
Fingerprint
Keywords
- Conjugated linoleic acid
- Leptin
- Lipid accumulation
- Matrix metalloproteinase-2
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid. / Moon, Hyun-Seuk; Lee, Hong Gu; Seo, Ji Hye; Chung, Chung Soo; Guo, Ding Ding; Kim, Tae Gyu; Choi, Yun Jaie; Cho, Chong Su.
In: Biochemical and Biophysical Research Communications, Vol. 356, No. 4, 18.05.2007, p. 955-960.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid
AU - Moon, Hyun-Seuk
AU - Lee, Hong Gu
AU - Seo, Ji Hye
AU - Chung, Chung Soo
AU - Guo, Ding Ding
AU - Kim, Tae Gyu
AU - Choi, Yun Jaie
AU - Cho, Chong Su
PY - 2007/5/18
Y1 - 2007/5/18
N2 - It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.
AB - It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.
KW - Conjugated linoleic acid
KW - Leptin
KW - Lipid accumulation
KW - Matrix metalloproteinase-2
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UR - http://www.scopus.com/inward/citedby.url?scp=34047097866&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.03.068
DO - 10.1016/j.bbrc.2007.03.068
M3 - Article
VL - 356
SP - 955
EP - 960
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 4
ER -