Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid

Hyun Seuk Moon, Hong Gu Lee, Ji Hye Seo, Chung Soo Chung, Ding Ding Guo, Tae Gyu Kim, Yun Jaie Choi, Chong Su Cho

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.

Original languageEnglish
Pages (from-to)955-960
Number of pages6
JournalBiochemical and biophysical research communications
Volume356
Issue number4
DOIs
Publication statusPublished - 2007 May 18

Keywords

  • Conjugated linoleic acid
  • Leptin
  • Lipid accumulation
  • Matrix metalloproteinase-2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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