TY - JOUR
T1 - Leptin-induced matrix metalloproteinase-2 secretion is suppressed by trans-10,cis-12 conjugated linoleic acid
AU - Moon, Hyun Seuk
AU - Lee, Hong Gu
AU - Seo, Ji Hye
AU - Chung, Chung Soo
AU - Guo, Ding Ding
AU - Kim, Tae Gyu
AU - Choi, Yun Jaie
AU - Cho, Chong Su
N1 - Funding Information:
This work was supported by the Agricultural R&D Promotion Center (ARPC) (2006-0053). We thank the National Instrumentation Center for Environmental Management (NICEM) and Research Institute of Pharmaceutical Sciences (RIPS) for measurement of PKC activation, ROS production, and NF-κB activation. We also thank Prof. Byung-Hoon Lee for technical assistance. H.S. Moon and J.H. Seo were supported by Brain Korea 21 (BK21) grant.
PY - 2007/5/18
Y1 - 2007/5/18
N2 - It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.
AB - It has long been recognized that leptin, a hormone made by adipocytes, is an important circulating signal for the regulation of body weight. In addition, matrix metalloproteinase (MMP), especially MMP-2, an adipocyte-secreted protein which promotes multi-cellular adipose clusters, is up-regulated in obesity. The present study is designed to evaluate whether trans-10,cis-12 conjugated linoleic acid (t-CLA) can suppress leptin-induced MMP-2 secretion in 3T3-L1 cells. The result showed that expressions of adipocyte marker proteins were significantly reduced by t-CLA-treated cultures, but not by linoleic acid (LA)-treated ones. Interestingly, MMP-2 secretion was significantly increased by leptin-treated cultures, thereby leading to accelerate adipocyte differentiation, indicating that MMP-2 was a necessary mediator of adipogenesis. However, increasing concentration of t-CLA significantly reduced leptin-induced MMP-2 secretion and triglyceride (TG) content. These findings provide support for a role for t-CLA in the regulation of metabolism in leptin-induced adipose tissue development.
KW - Conjugated linoleic acid
KW - Leptin
KW - Lipid accumulation
KW - Matrix metalloproteinase-2
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U2 - 10.1016/j.bbrc.2007.03.068
DO - 10.1016/j.bbrc.2007.03.068
M3 - Article
C2 - 17386921
AN - SCOPUS:34047097866
VL - 356
SP - 955
EP - 960
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -
