Leptin is a dose-dependent marker of caloric restriction in adipose tissues located in different parts of the mouse body

Seok Jin Hong, Seung Soo Kim, Whasun Lim, Gwonhwa Song, Cheol-Koo Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)


We investigated leptin expression in four fat tissues located in different body parts of male mice. In the absence of caloric restriction (CR), the highest relative levels of leptin mRNA were detected in epididymal fat (EF). Lateral abdominal fat (LAF) and subcutaneous fat (SF) had intermediate leptin expression levels, while the lowest leptin mRNA content was revealed in brown adipose tissue (BAT). We also investigated differential regulation of leptin expression in these adipose tissues following diets that were based on various levels of CR for 10 weeks. The largest reduction of leptin mRNA levels by CR was observed in adipose tissue where maximal leptin mRNA expression was detected in the absence of CR. Leptin protein expression was also decreased by CR as revealed by immunohistochemistry. The changes in leptin mRNA and protein expression positively correlated with adipocyte size that was also diminished with the increase in the strength of CR.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalMolecular and Cellular Toxicology
Issue number1
Publication statusPublished - 2018 Jan 1



  • Adipocyte
  • Adipose tissue
  • Caloric restriction
  • Leptin
  • Mouse

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this