Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells

Je Sang Ko, In Sik Kim, Sung W. Jang, Young Han Lee, Soon Young Shin, Do Sik Min, Doe Sun Na

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Leukotactin-1 (Lkn-1)/CCL15 is a recently cloned CC-chemokine that binds to the CCR1 and CCR3. Although Lkn-1 has been known to function as a chemoattractant for neutrophils, monocytes and lymphocytes, its cellular mechanism remains unclear. To understand the mechanism of Lkn-1-induced chemotaxis signaling, we examined the chemotactic activities of human osteogenic sarcoma cells expressing CCR1 in response to Lkn-1 using inhibitors of signaling molecules. Inhibitors of Gi/Go protein, phospholipase C (PLC) and protein kinase Cδ (PKCδ) inhibited the chemotactic activity of Lkn-1 indicating that Lkn-1-induced chemotaxis signal is transduced through Gi/Go protein, PLC and PKCδ. The activities of PLC and PKCδ were also enhanced by Lkn-1 stimulation. Chemotactic activity of Lkn-1 was inhibited by the treatment of cycloheximide and actinomycin D suggesting that newly synthesized proteins are needed for chemotaxis. Nuclear factor-κB (NF-κB) inhibitor reduced chemotactic activity of Lkn-1. DNA binding activity of NF-κB was also enhanced by Lkn-1 stimulation. These results suggest that Lkn-1 transduces the signal through Gi/Go protein, PLC, PKCδ, NF-κB and newly synthesized proteins for chemotaxis.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalFEBS Letters
Volume515
Issue number1-3
DOIs
Publication statusPublished - 2002 Mar 27
Externally publishedYes

Fingerprint

Type C Phospholipases
Chemotaxis
Protein Kinase C
Complement Factor B
Protein C
Proteins
CC Chemokines
Chemotactic Factors
Dactinomycin
Osteosarcoma
Cycloheximide
Human Activities
Lymphocytes
Monocytes
Neutrophils
Cells
DNA
Molecules
Therapeutics

Keywords

  • Chemokine
  • Nuclear factor-κB
  • Phospholipase C
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ko, J. S., Kim, I. S., Jang, S. W., Lee, Y. H., Shin, S. Y., Min, D. S., & Na, D. S. (2002). Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells. FEBS Letters, 515(1-3), 159-164. https://doi.org/10.1016/S0014-5793(02)02465-1

Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells. / Ko, Je Sang; Kim, In Sik; Jang, Sung W.; Lee, Young Han; Shin, Soon Young; Min, Do Sik; Na, Doe Sun.

In: FEBS Letters, Vol. 515, No. 1-3, 27.03.2002, p. 159-164.

Research output: Contribution to journalArticle

Ko, JS, Kim, IS, Jang, SW, Lee, YH, Shin, SY, Min, DS & Na, DS 2002, 'Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells', FEBS Letters, vol. 515, no. 1-3, pp. 159-164. https://doi.org/10.1016/S0014-5793(02)02465-1
Ko, Je Sang ; Kim, In Sik ; Jang, Sung W. ; Lee, Young Han ; Shin, Soon Young ; Min, Do Sik ; Na, Doe Sun. / Leukotactin-1/CCL15-induced chemotaxis signaling through CCR1 in HOS cells. In: FEBS Letters. 2002 ; Vol. 515, No. 1-3. pp. 159-164.
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