Levobupivacaine-induced contraction of isolated rat aorta is calcium dependent

Ji Seok Baik, Ju Tae Sohn, Seong Ho Ok, Jae Gak Kim, Hui Jin Sung, Sang Seung Park, Jae-Yong Park, Eun Mi Hwang, Young Kyun Chung

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Levobupivacaine is a long-acting local anesthetic that intrinsically produces vasoconstriction in isolated vessels. The goals of this study were to investigate the calcium-dependent mechanism underlying levobupivacaine-induced contraction of isolated rat aorta in vitro and to elucidate the pathway responsible for the endothelium-dependent attenuation of levobupivacaine-induced contraction. Isolated rat aortic rings were suspended to record isometric tension. Cumulative levobupivacaine concentration-response curves were generated in either the presence or absence of the antagonists verapamil, nifedipine, SKF-96365, 2-aminoethoxydiphenylborate, Gd 3+ , N W -nitro-L-arginine methyl ester (L-NAME), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), and methylene blue, either alone or in combination. Verapamil, nifedipine, SKF-96365, 2-aminoethoxydiphenylborate, low calcium concentrations, and calcium-free Krebs solution attenuated levobupivacaine-induced contraction. Gd 3+ had no effect on levobupivacaine-induced contraction. Levobupivacaine increased intracellular calcium levels in vascular smooth muscle cells. L-NAME, ODQ, and methylene blue increased levobupivacaine-induced contraction in endothelium-intact aorta. SKF-96365 attenuated calcium-induced contraction in a previously calcium-free isotonic depolarizing solution containing 100 mmol/L KCl. Levobupivacaine-induced contraction of rat aortic smooth muscle is mediated primarily by calcium influx from the extracellular space mainly via voltage-operated calcium channels and, in part, by inositol 1,4,5-trisphosphate receptor-mediated release of calcium from the sarcoplasmic reticulum. The nitric oxide - cyclic guanosine monophosphate pathway is involved in the endothelium-dependent attenuation of levobupivacaine-induced contraction.

Original languageEnglish
Pages (from-to)467-476
Number of pages10
JournalCanadian Journal of Physiology and Pharmacology
Volume89
Issue number7
DOIs
Publication statusPublished - 2011 Jul 1
Externally publishedYes

Fingerprint

Aorta
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Calcium
Endothelium
Methylene Blue
NG-Nitroarginine Methyl Ester
Nifedipine
Verapamil
levobupivacaine
Isotonic Solutions
Inositol 1,4,5-Trisphosphate Receptors
Cyclic GMP
Extracellular Space
Sarcoplasmic Reticulum
Calcium Channels
Local Anesthetics
Vasoconstriction
Vascular Smooth Muscle
Smooth Muscle Myocytes
Smooth Muscle

Keywords

  • Aorta
  • Calcium
  • Contraction
  • Endothelium
  • Levobupivacaine
  • Nitric oxide
  • Verapamil

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

Cite this

Baik, J. S., Sohn, J. T., Ok, S. H., Kim, J. G., Sung, H. J., Park, S. S., ... Chung, Y. K. (2011). Levobupivacaine-induced contraction of isolated rat aorta is calcium dependent. Canadian Journal of Physiology and Pharmacology, 89(7), 467-476. https://doi.org/10.1139/Y11-046

Levobupivacaine-induced contraction of isolated rat aorta is calcium dependent. / Baik, Ji Seok; Sohn, Ju Tae; Ok, Seong Ho; Kim, Jae Gak; Sung, Hui Jin; Park, Sang Seung; Park, Jae-Yong; Hwang, Eun Mi; Chung, Young Kyun.

In: Canadian Journal of Physiology and Pharmacology, Vol. 89, No. 7, 01.07.2011, p. 467-476.

Research output: Contribution to journalArticle

Baik, JS, Sohn, JT, Ok, SH, Kim, JG, Sung, HJ, Park, SS, Park, J-Y, Hwang, EM & Chung, YK 2011, 'Levobupivacaine-induced contraction of isolated rat aorta is calcium dependent', Canadian Journal of Physiology and Pharmacology, vol. 89, no. 7, pp. 467-476. https://doi.org/10.1139/Y11-046
Baik, Ji Seok ; Sohn, Ju Tae ; Ok, Seong Ho ; Kim, Jae Gak ; Sung, Hui Jin ; Park, Sang Seung ; Park, Jae-Yong ; Hwang, Eun Mi ; Chung, Young Kyun. / Levobupivacaine-induced contraction of isolated rat aorta is calcium dependent. In: Canadian Journal of Physiology and Pharmacology. 2011 ; Vol. 89, No. 7. pp. 467-476.
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