Levosulpiride, (S)-(-)-5-Aminosulfonyl-N-[(1-ethyl-2-pyrrolidinyl) methyl]-2-methoxybenzamide, enhances the transduction efficiency of PEP-1-ribosomal protein S3 in vitro and in vivo

Eun Hee Ahn, Dae Won Kim, Duk Soo Kim, Su Jung Woo, Hye Ri Kim, Joon Kim, Soon Sung Lim, Tae Cheon Kang, Dong Joon Kim, Ki Tae Suk, Jinseu Park, Qiuxiang Luo, Won Sik Eum, Hyun Sook Hwang, Soo Young Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Many proteins with poor transduction efficiency were reported to be delivered to cells by fusion with protein transduction domains (PTDs). In this study, we investigated the effect of levosulpiride on the transduction of PEP-1 ribosomal protein S3 (PEP-1-rpS3), and examined its influence on the stimulation of the therapeutic properties of PEP-1-rpS3. PEP-1-rpS3 transduction into HaCaT human keratinocytes and mouse skin was stimulated by levosulpiride in a manner that did not directly affect the cell viability. Following 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice, levosulpiride alone was ineffective in reducing TPA-induced edema and in inhibiting the elevated productions of inflammatory mediators and cytokines, such as cyclooxygenase-2, inducible nitric oxide synthase, interleukin-6 and -1β, and tumor necrosis factor-α. Anti-inflammatory activity by PEP-1-rpS3 + levosulpiride was significantly more potent than by PEP-1-rpS3 alone. These results suggest that levosulpiride may be useful for enhancing the therapeutic effect of PEP-1-rpS3 against various inflammatory diseases.

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalBMB Reports
Volume44
Issue number5
DOIs
Publication statusPublished - 2011 May 1

Fingerprint

Ribosomal Proteins
Acetates
Tetradecanoylphorbol Acetate
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Interleukin-6
Skin
Cell Fusion
Proteins
Anti-Inflammatory Agents
Fusion reactions
Tumor Necrosis Factor-alpha
Therapeutic Uses
Cells
Peptamen
In Vitro Techniques
levosulpiride
Interleukin-1
Keratinocytes
Cytokines

Keywords

  • Inflammation
  • Levosulpiride
  • PEP-1-rpS3
  • Protein therapy
  • Protein transduction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Levosulpiride, (S)-(-)-5-Aminosulfonyl-N-[(1-ethyl-2-pyrrolidinyl) methyl]-2-methoxybenzamide, enhances the transduction efficiency of PEP-1-ribosomal protein S3 in vitro and in vivo. / Ahn, Eun Hee; Kim, Dae Won; Kim, Duk Soo; Woo, Su Jung; Kim, Hye Ri; Kim, Joon; Lim, Soon Sung; Kang, Tae Cheon; Kim, Dong Joon; Suk, Ki Tae; Park, Jinseu; Luo, Qiuxiang; Eum, Won Sik; Hwang, Hyun Sook; Choi, Soo Young.

In: BMB Reports, Vol. 44, No. 5, 01.05.2011, p. 329-334.

Research output: Contribution to journalArticle

Ahn, EH, Kim, DW, Kim, DS, Woo, SJ, Kim, HR, Kim, J, Lim, SS, Kang, TC, Kim, DJ, Suk, KT, Park, J, Luo, Q, Eum, WS, Hwang, HS & Choi, SY 2011, 'Levosulpiride, (S)-(-)-5-Aminosulfonyl-N-[(1-ethyl-2-pyrrolidinyl) methyl]-2-methoxybenzamide, enhances the transduction efficiency of PEP-1-ribosomal protein S3 in vitro and in vivo', BMB Reports, vol. 44, no. 5, pp. 329-334. https://doi.org/10.5483/BMBRep.2011.44.5.329
Ahn, Eun Hee ; Kim, Dae Won ; Kim, Duk Soo ; Woo, Su Jung ; Kim, Hye Ri ; Kim, Joon ; Lim, Soon Sung ; Kang, Tae Cheon ; Kim, Dong Joon ; Suk, Ki Tae ; Park, Jinseu ; Luo, Qiuxiang ; Eum, Won Sik ; Hwang, Hyun Sook ; Choi, Soo Young. / Levosulpiride, (S)-(-)-5-Aminosulfonyl-N-[(1-ethyl-2-pyrrolidinyl) methyl]-2-methoxybenzamide, enhances the transduction efficiency of PEP-1-ribosomal protein S3 in vitro and in vivo. In: BMB Reports. 2011 ; Vol. 44, No. 5. pp. 329-334.
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AU - Kim, Hye Ri

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AU - Lim, Soon Sung

AU - Kang, Tae Cheon

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AU - Suk, Ki Tae

AU - Park, Jinseu

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