Ligation of ICAM-1 molecules inhibits target cell-induced granule exocytosis of IL-12-activated natural killer cells

D. H. Cho, H. K. Song, H. S. Kang, S. R. Yoon, H. G. Lee, K. H. Pyun, W. J. Lee, Y. B. Kim, I. Choi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The importance of cell adhesion molecules such as ICAM-1 is emphasized in cell-to-cell interactions that are critical in the generation of effective immune reactions. In this study, the involvement of ICAM-1 in natural killer (NK) cell activities was characterized in IL-12-activated human NK cells. To address the question of whether ligation of ICAM-1 molecules can modulate NK cell cytolytic activities, a 4-h 51Cr-release assay was performed after pretreatment of NK cells with R6.5 mAb (anti-human ICAM-1 mAb). Ligation of membrane ICAM-1 molecules significantly inhibited IL-12-enhanced NK cytotoxicity against K562, and the pretreatment of neutralizing soluble ICAM- 1 with R6.5 mAb blocked this inhibitory effect. The involvement of Ca2+- dependent granular exocytosis was evaluated. BLT esterase assay demonstrated that the ligation of ICAM-1 molecules inhibited granular exocytosis of NK cells. Additionally, the ICAM-1-mediated inhibition of Ca2+ flux in NK cells was detected using Fluo-3AM, while the pretreatment of NK cells with R6.5 mAb did not affect conjugate formation between NK and K562 cells. Collectively, these results suggest that the signals transduced from ICAM-1 molecules might be sufficient to induce inhibitory effects on NK cells. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalCellular Immunology
Volume199
Issue number1
DOIs
Publication statusPublished - 2000 Jan 10
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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