Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling

Ki Young Chang; Doyeon Woo; Hyunjin Jung; Sangkyu Lee; Sungsoo Kim; Joungha Won; Taeyoon Kyung; Hyerim Park; Nury Kim; Hee Won Yang; Jae Yong Park; Eun Mi Hwang; Daesoo Kim; Won Do Heo

doi:10.1038/ncomms5057

Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling

Ki Young Chang, Doyeon Woo, Hyunjin Jung, Sangkyu Lee, Sungsoo Kim, Joungha Won, Taeyoon Kyung, Hyerim Park, Nury Kim, Hee Won Yang, Jae Yong Park, Eun Mi Hwang, Daesoo Kim, Won Do Heo

Research output: Contribution to journal › Article

70 Citations (Scopus)

Abstract

Receptor tyrosine kinases (RTKs) are a family of cell-surface receptors that have a key role in regulating critical cellular processes. Here, to understand and precisely control RTK signalling, we report the development of a genetically encoded, photoactivatable Trk (tropomyosin-related kinase) family of RTKs using a light-responsive module based on Arabidopsis thaliana cryptochrome 2. Blue-light stimulation (488-nm) of mammalian cells harbouring these receptors robustly upregulates canonical Trk signalling. A single light stimulus triggers transient signalling activation, which is reversibly tuned by repetitive delivery of blue-light pulses. In addition, the light-provoked process is induced in a spatially restricted and cell-specific manner. A prolonged patterned illumination causes sustained activation of extracellular signal-regulated kinase and promotes neurite outgrowth in a neuronal cell line, and induces filopodia formation in rat hippocampal neurons. These light-controllable receptors are expected to create experimental opportunities to spatiotemporally manipulate many biological processes both in vitro and in vivo.

Original language	English
Article number	4057
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms5057
Publication status	Published - 2014 Jun 4
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Chang, K. Y., Woo, D., Jung, H., Lee, S., Kim, S., Won, J., Kyung, T., Park, H., Kim, N., Yang, H. W., Park, J. Y., Hwang, E. M., Kim, D., & Do Heo, W. (2014). Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling. Nature communications, 5, [4057]. https://doi.org/10.1038/ncomms5057

Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling. / Chang, Ki Young; Woo, Doyeon; Jung, Hyunjin; Lee, Sangkyu; Kim, Sungsoo; Won, Joungha; Kyung, Taeyoon; Park, Hyerim; Kim, Nury; Yang, Hee Won; Park, Jae Yong; Hwang, Eun Mi; Kim, Daesoo; Do Heo, Won.

In: Nature communications, Vol. 5, 4057, 04.06.2014.

Research output: Contribution to journal › Article

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abstract = "Receptor tyrosine kinases (RTKs) are a family of cell-surface receptors that have a key role in regulating critical cellular processes. Here, to understand and precisely control RTK signalling, we report the development of a genetically encoded, photoactivatable Trk (tropomyosin-related kinase) family of RTKs using a light-responsive module based on Arabidopsis thaliana cryptochrome 2. Blue-light stimulation (488-nm) of mammalian cells harbouring these receptors robustly upregulates canonical Trk signalling. A single light stimulus triggers transient signalling activation, which is reversibly tuned by repetitive delivery of blue-light pulses. In addition, the light-provoked process is induced in a spatially restricted and cell-specific manner. A prolonged patterned illumination causes sustained activation of extracellular signal-regulated kinase and promotes neurite outgrowth in a neuronal cell line, and induces filopodia formation in rat hippocampal neurons. These light-controllable receptors are expected to create experimental opportunities to spatiotemporally manipulate many biological processes both in vitro and in vivo.",

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