Limnobaculum parvum gen. nov., sp. nov., isolated from a freshwater lake

Chaeyun Baek, Su Kyoung Shin, Hana Yi

Research output: Contribution to journalArticle

Abstract

A Gram-stain-negative, rod-shaped, facultatively anaerobic bacterium, designated as strain HYN0051T, was isolated from lake water. 16S rRNA gene sequence analyses indicated that the isolate shares the highest sequence similarity with the genus Pragia (97.1 %) of the family Enterobacteriaceae. Strain HYN0051T did not form a rigid clade with the genus Pragia in any of the phylogenetic trees, demonstrating the novel generic status of the isolate. The genome of strain HYN0051T (CP029185) is a single circular chromosome of 3.84 Mb. The major fatty acids detected were C14 : 0, C16 : 0, summed feature 2 (C12 : 0 aldehyde and/or unknown 10.928), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The isoprenoid quinones were Q-8, MK-8 and DMK-8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid. The genome size, G+C content and fatty acid profile were very similar to that of the genus Pragia, but the composition of isoprenoid quinones and polar lipids, and numerous phenotypic properties also supported the distinctiveness of the new isolate from the genus Pragia. Thus, based on phylogenetic and phenotypic data, a novel species of a new genus, Limnobaculum parvum gen. nov., sp. nov., is proposed. The type strain of Limnobaculum parvum is HYN0051T (=KACC 19186T=NBRC 112742T).

Original languageEnglish
Pages (from-to)1826-1830
Number of pages5
JournalInternational journal of systematic and evolutionary microbiology
Volume69
Issue number6
DOIs
Publication statusPublished - 2019 Jun 1

Fingerprint

Pragia
Quinones
isoprenoid
Terpenes
Lakes
Fresh Water
Fatty Acids
fatty acid
genome
lipid
phylogenetics
Lipids
Genome Size
lakes
Phosphatidylglycerols
Anaerobic Bacteria
new species
isoprenoids
Base Composition
lake

Keywords

  • lake water
  • Limnobaculum
  • Nakdong River

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Limnobaculum parvum gen. nov., sp. nov., isolated from a freshwater lake. / Baek, Chaeyun; Shin, Su Kyoung; Yi, Hana.

In: International journal of systematic and evolutionary microbiology, Vol. 69, No. 6, 01.06.2019, p. 1826-1830.

Research output: Contribution to journalArticle

@article{f9ef4a73ed24490b8ee6c7f6be62e019,
title = "Limnobaculum parvum gen. nov., sp. nov., isolated from a freshwater lake",
abstract = "A Gram-stain-negative, rod-shaped, facultatively anaerobic bacterium, designated as strain HYN0051T, was isolated from lake water. 16S rRNA gene sequence analyses indicated that the isolate shares the highest sequence similarity with the genus Pragia (97.1 {\%}) of the family Enterobacteriaceae. Strain HYN0051T did not form a rigid clade with the genus Pragia in any of the phylogenetic trees, demonstrating the novel generic status of the isolate. The genome of strain HYN0051T (CP029185) is a single circular chromosome of 3.84 Mb. The major fatty acids detected were C14 : 0, C16 : 0, summed feature 2 (C12 : 0 aldehyde and/or unknown 10.928), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The isoprenoid quinones were Q-8, MK-8 and DMK-8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid. The genome size, G+C content and fatty acid profile were very similar to that of the genus Pragia, but the composition of isoprenoid quinones and polar lipids, and numerous phenotypic properties also supported the distinctiveness of the new isolate from the genus Pragia. Thus, based on phylogenetic and phenotypic data, a novel species of a new genus, Limnobaculum parvum gen. nov., sp. nov., is proposed. The type strain of Limnobaculum parvum is HYN0051T (=KACC 19186T=NBRC 112742T).",
keywords = "lake water, Limnobaculum, Nakdong River",
author = "Chaeyun Baek and Shin, {Su Kyoung} and Hana Yi",
year = "2019",
month = "6",
day = "1",
doi = "10.1099/ijsem.0.003401",
language = "English",
volume = "69",
pages = "1826--1830",
journal = "International Journal of Systematic Bacteriology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "6",

}

TY - JOUR

T1 - Limnobaculum parvum gen. nov., sp. nov., isolated from a freshwater lake

AU - Baek, Chaeyun

AU - Shin, Su Kyoung

AU - Yi, Hana

PY - 2019/6/1

Y1 - 2019/6/1

N2 - A Gram-stain-negative, rod-shaped, facultatively anaerobic bacterium, designated as strain HYN0051T, was isolated from lake water. 16S rRNA gene sequence analyses indicated that the isolate shares the highest sequence similarity with the genus Pragia (97.1 %) of the family Enterobacteriaceae. Strain HYN0051T did not form a rigid clade with the genus Pragia in any of the phylogenetic trees, demonstrating the novel generic status of the isolate. The genome of strain HYN0051T (CP029185) is a single circular chromosome of 3.84 Mb. The major fatty acids detected were C14 : 0, C16 : 0, summed feature 2 (C12 : 0 aldehyde and/or unknown 10.928), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The isoprenoid quinones were Q-8, MK-8 and DMK-8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid. The genome size, G+C content and fatty acid profile were very similar to that of the genus Pragia, but the composition of isoprenoid quinones and polar lipids, and numerous phenotypic properties also supported the distinctiveness of the new isolate from the genus Pragia. Thus, based on phylogenetic and phenotypic data, a novel species of a new genus, Limnobaculum parvum gen. nov., sp. nov., is proposed. The type strain of Limnobaculum parvum is HYN0051T (=KACC 19186T=NBRC 112742T).

AB - A Gram-stain-negative, rod-shaped, facultatively anaerobic bacterium, designated as strain HYN0051T, was isolated from lake water. 16S rRNA gene sequence analyses indicated that the isolate shares the highest sequence similarity with the genus Pragia (97.1 %) of the family Enterobacteriaceae. Strain HYN0051T did not form a rigid clade with the genus Pragia in any of the phylogenetic trees, demonstrating the novel generic status of the isolate. The genome of strain HYN0051T (CP029185) is a single circular chromosome of 3.84 Mb. The major fatty acids detected were C14 : 0, C16 : 0, summed feature 2 (C12 : 0 aldehyde and/or unknown 10.928), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The isoprenoid quinones were Q-8, MK-8 and DMK-8. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid. The genome size, G+C content and fatty acid profile were very similar to that of the genus Pragia, but the composition of isoprenoid quinones and polar lipids, and numerous phenotypic properties also supported the distinctiveness of the new isolate from the genus Pragia. Thus, based on phylogenetic and phenotypic data, a novel species of a new genus, Limnobaculum parvum gen. nov., sp. nov., is proposed. The type strain of Limnobaculum parvum is HYN0051T (=KACC 19186T=NBRC 112742T).

KW - lake water

KW - Limnobaculum

KW - Nakdong River

UR - http://www.scopus.com/inward/record.url?scp=85067089809&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067089809&partnerID=8YFLogxK

U2 - 10.1099/ijsem.0.003401

DO - 10.1099/ijsem.0.003401

M3 - Article

C2 - 30994436

AN - SCOPUS:85067089809

VL - 69

SP - 1826

EP - 1830

JO - International Journal of Systematic Bacteriology

JF - International Journal of Systematic Bacteriology

SN - 1466-5026

IS - 6

ER -