Limonene, a natural cyclic terpene, is an agonistic ligand for adenosine A2A receptors

Hyo Min Park, Ji Hae Lee, Jia Yaoyao, Hee Jin Jun, Sung-Joon Lee

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Limonene is a major aromatic compound in essential oils extracted from citrus rind. The application of limonene, especially in aromatherapy, has expanded significantly, but its potential effects on cellular metabolism have been elusive. We found that limonene directly binds to the adenosine A2A receptor, which may induce sedative effects. Results from an in vitro radioligand binding assay showed that limonene exhibits selective affinity to A2A receptors. In addition, limonene increased cytosolic cAMP concentration and induced activation of protein kinase A and phosphorylation of cAMP-response element-binding protein in Chinese hamster ovary cells transfected with the human adenosine A2A receptor gene. Limonene also increased cytosolic calcium concentration, which can be achieved by the activation of adenosine A2A receptors. These findings suggest that limonene can act as a ligand and an agonist for adenosine A2A receptors.

Original languageEnglish
Pages (from-to)345-348
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume404
Issue number1
DOIs
Publication statusPublished - 2011 Jan 7

Fingerprint

Adenosine A2A Receptors
Terpenes
Ligands
Adenosine A2 Receptor Agonists
Chemical activation
Aromatherapy
Cyclic AMP Response Element-Binding Protein
Radioligand Assay
Phosphorylation
Aromatic compounds
Citrus
Volatile Oils
Cyclic AMP-Dependent Protein Kinases
Cricetulus
limonene
Hypnotics and Sedatives
Metabolism
Ovary
Assays
Genes

Keywords

  • Adenosine A receptors
  • CAMP
  • Limonene
  • Radioligand binding assay

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Limonene, a natural cyclic terpene, is an agonistic ligand for adenosine A2A receptors. / Park, Hyo Min; Lee, Ji Hae; Yaoyao, Jia; Jun, Hee Jin; Lee, Sung-Joon.

In: Biochemical and Biophysical Research Communications, Vol. 404, No. 1, 07.01.2011, p. 345-348.

Research output: Contribution to journalArticle

Park, Hyo Min ; Lee, Ji Hae ; Yaoyao, Jia ; Jun, Hee Jin ; Lee, Sung-Joon. / Limonene, a natural cyclic terpene, is an agonistic ligand for adenosine A2A receptors. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 404, No. 1. pp. 345-348.
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